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产品描述：人胞外超氧化物歧化酶(SOD3)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆、唾液、尿液样本中的SOD3含量。SOD3是一种超氧化物歧化酶，编码于SOD3基因，属于SOD蛋白家族。它主要在细胞外发挥作用，通过催化超氧阴离子转化为氧气和过氧化氢，保护脑、肺等组织免受氧化应激。SOD3的研究机制涉及其在免疫调节、抗氧化应激中的作用，以及与多种疾病的关联。试剂盒检测范围为7.8 pg/mL-500 pg/mL，本产品为氧化应激机制研究、疾病模型构建及药物筛选等科研领域提供可靠工具，特别适用于探究生物体液或细胞微环境中SOD3的动态变化及其与病理进程的关联性研究。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：EC SOD ELISA Kit; EC-SOD ELISA Kit; Extracellular superoxide dismutase [Cu Zn] ELISA Kit; Extracellular superoxide dismutase [Cu-Zn] ELISA Kit; Extracellular superoxide dismutase ELISA Kit; Extracellular superoxide dismutase precursor ELISA Kit; MGC20077 ELISA Kit; SOD 3 ELISA Kit; SOD3 ELISA Kit; SODE_HUMAN ELISA Kit; Superoxide Dismutase 3 ELISA Kit; Superoxide dismutase 3 extracellular ELISA Kit
缩写：SOD3
Uniprot No.：P08294
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates, tissue homogenates, saliva, urine
检测范围：7.8 pg/mL-500 pg/mL
灵敏度：1.95 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human SOD3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1000 | Average % | 90 | ||||
| Range % | 82-96 | |||||
| 1:2000 | Average % | 95 | ||||
| Range % | 90-102 | |||||
| 1:4000 | Average % | 98 | ||||
| Range % | 92-105 | |||||
| 1:8000 | Average % | 87 | ||||
| Range % | 83-93 | |||||
回收率：
| The recovery of human SOD3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 87-96 | ||||
| EDTA plasma (n=4) | 95 | 92-102 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected5002.7722.7472.7602.6242502.3492.3792.3642.2281251.7121.6941.7031.56762.51.0560.9931.0250.88931.20.5910.5520.5720.43615.60.3720.3640.3680.2327.80.2250.2170.2210.08500.1340.1380.136 | ||||||
货期：3-5 working days
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