| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | p110-RB1; pRb; pp105 |
| Entrez GeneID | 19645 |
| WB Predicted band size | Calculated MW: 105 kDa; Observed MW: 105 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Rat |
| Immunogen | Recombinant protein of mouse Rb |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于 **Rb抗体** 的模拟参考文献示例，内容基于常见研究主题，供参考：
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1. **文献名称**: "Validation of a Phospho-Specific Rb Antibody for Monitoring Cell Cycle Dynamics"
**作者**: Johnson R, et al.
**摘要**: 该研究验证了一种针对Rb蛋白磷酸化位点(Ser780)的特异性抗体，通过Western blot和流式细胞术分析，证明其在细胞周期调控研究中的应用，尤其是在G1/S期转换中的检测效果。
2. **文献名称**: "Rb Antibody Application in Tumor Suppression Studies"
**作者**: Chen L, et al.
**摘要**: 利用Rb抗体进行免疫组化分析，发现Rb蛋白在多种肿瘤组织中的表达缺失与癌症进展相关，提示其作为肿瘤抑制标志物的潜在价值。
3. **文献名称**: "Comparative Analysis of Commercial Rb Antibodies in Immunoprecipitation Assays"
**作者**: Gupta S, et al.
**摘要**: 对比了不同厂商的Rb抗体在免疫共沉淀(Co-IP)中的性能，评估了其特异性及与E2F转录因子结合检测的可靠性，为下游功能研究提供参考。
4. **文献名称**: "Role of Rb in Cellular Senescence: Insights from Antibody-Mediated Knockdown"
**作者**: Martinez P, et al.
**摘要**: 通过Rb抗体的siRNA干扰实验，证实Rb蛋白在细胞衰老过程中的关键作用，并揭示了其与p53通路协同调控的机制。
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**注**：以上文献为示例，非真实存在。如需真实文献，建议通过PubMed、Google Scholar等平台检索关键词(如“Rb antibody application”或“Retinoblastoma protein antibody”)获取。
Rb antibodies specifically target the retinoblastoma protein (pRb), a critical tumor suppressor encoded by the RB1 gene. Discovered in the context of retinoblastoma, a rare childhood eye cancer, pRb plays a central role in regulating the cell cycle by controlling the G1-to-S phase transition. It inhibits cell proliferation by binding to E2F transcription factors, thereby blocking the expression of genes required for DNA replication. Dysfunctional pRb, due to mutations or inactivation (e.g., phosphorylation by cyclin-dependent kinases), disrupts this regulatory mechanism, leading to uncontrolled cell division and tumorigenesis.
Rb antibodies are widely used in research and diagnostics to study pRb expression, phosphorylation status, and interactions in cancer biology. They help identify RB1 gene inactivation events, assess tumor suppressor activity, and explore therapeutic targets. In clinical settings, these antibodies aid in immunohistochemical analyses to correlate pRb loss with cancer progression or treatment resistance. Additionally, Rb antibodies contribute to understanding developmental biology, as pRb also influences differentiation and apoptosis. Their applications span Western blotting, immunofluorescence, and flow cytometry, making them essential tools for investigating cell cycle regulation and oncogenic pathways.
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