首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠锰超氧化物歧化酶，线粒体(SOD2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的SOD2含量。SOD2是超氧化物歧化酶2，定位于线粒体中。它能催化超氧阴离子发生歧化反应，清除细胞内过多的超氧阴离子，减少氧化应激损伤。其异常表达与多种疾病的发生发展相关，如神经退行性疾病、心血管疾病等，是研究疾病机制和治疗的潜在靶点。试剂盒检测范围为0.625 ng/mL-40 ng/mL，科研人员可通过定量评估小鼠模型在氧化应激相关疾病（如糖尿病、帕金森病）中的SOD2动态变化，或用于基础研究中线粒体功能调控机制探索。试剂盒操作流程标准化，配备完整反应组分，支持在实验室常规条件下开展细胞、组织或体液样本的SOD2水平检测，为抗氧化治疗、疾病机制研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Sod2 ELISA Kit; Sod-2 ELISA Kit; Superoxide dismutase [Mn] ELISA Kit; mitochondrial ELISA Kit; EC 1.15.1.1 ELISA Kit
缩写：SOD2
Uniprot No.：P09671
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：0.625 ng/mL-40 ng/mL
灵敏度：0.156 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse SOD2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 95 | ||||
| Range % | 86-98 | |||||
| 1:2 | Average % | 100 | ||||
| Range % | 97-104 | |||||
| 1:4 | Average % | 89 | ||||
| Range % | 85-93 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 91-100 | |||||
回收率：
| The recovery of mouse SOD2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 91 | 85-95 | ||||
| EDTA plasma (n=4) | 101 | 90-105 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected402.2922.2022.2472.064201.4151.4761.4461.263100.9560.9780.9670.78450.5480.5390.5440.3612.50.4480.4410.4450.2621.250.3420.3570.3500.1670.6250.2750.2660.2710.08800.1820.1840.183 | ||||||
货期：3-5 working days
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