| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CHUK; IKKA; TCF16; Inhibitor of nuclear factor kappa-B kinase subunit alpha; I-kappa-B kinase alpha; IKK-A; IKK-alpha; IkBKA; IkappaB kinase; Conserved helix-loop-helix ubiquitous kinase; I-kappa-B kinase 1; IKK1; Nuclear factor NF-kappa-B |
| Entrez GeneID | 1147 |
| WB Predicted band size | Calculated MW: 85 kDa; Observed MW: 85 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthetic peptide of human IKK alpha |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是3篇与IKKα抗体相关的经典文献摘要信息：
1. **文献名称**：*IKK-1 and IKK-2: Cytokine-Activated IκB Kinases Essential for NF-κB Activation*
**作者**：DiDonato, J.A. et al.
**摘要**：该研究首次鉴定了IKKα(IKK1)和IKKβ(IKK2)的分子结构，证明两者构成IKK复合体核心，通过磷酸化IκB蛋白激活NF-κB通路，并比较了二者在炎症信号传导中的功能差异。
2. **文献名称**：*Identification of a Novel Serine 176 Phosphorylation Site in IKKα Critical for Its Kinase Activity*
**作者**：Hu, Y. et al.
**摘要**：研究揭示了IKKα激酶活性依赖其Ser-176位点的磷酸化，并通过抗体检测验证了该修饰在TNFα信号中调控NF-κB非经典通路的作用。
3. **文献名称**：*Activation by IKKα of a Second, Evolutionary Conserved, NF-κB Signaling Pathway*
**作者**：Senftleben, U. et al.
**摘要**：文章提出IKKα独立于IKKβ激活NF-κB的替代通路，利用特异性抗体证明其在B细胞发育和淋巴组织生成中的独特功能。
4. **文献名称**：*IKKα Regulates Epidermal Differentiation through NF-κB and Non-NF-κB Pathways*
**作者**：Park, M.H. et al.
**摘要**：通过抗体介导的蛋白定位分析，发现IKKα通过调控靶基因(如kallikrein 7)影响皮肤分化，并参与乳腺癌转移的分子机制。
以上文献为IKKα功能与抗体应用提供了关键理论支持，涉及结构、修饰位点及多通路调控机制。
The IκB kinase (IKK) alpha antibody is a crucial tool for studying the IKK complex, a central regulator of the NF-κB signaling pathway. IKKα (IKK1), along with IKKβ (IKK2) and NEMO (IKKγ), forms the IKK complex, which phosphorylates IκB proteins to trigger their degradation, enabling NF-κB transcription factors to translocate into the nucleus and activate genes involved in inflammation, immunity, and cell survival. Unlike IKKβ, IKKα has distinct roles, including mediating non-canonical NF-κB signaling, regulating epidermal differentiation, and participating in developmental processes.
IKK alpha antibodies are designed to detect specific epitopes of the IKKα protein, enabling researchers to study its expression, post-translational modifications (e.g., phosphorylation at Ser176/180), and subcellular localization. These antibodies are widely used in techniques like Western blotting, immunoprecipitation, immunofluorescence, and immunohistochemistry. They are critical for investigating IKKα's involvement in diseases such as cancer, autoimmune disorders, and chronic inflammation.
Most IKK alpha antibodies are raised in rabbits or mice using recombinant protein fragments or synthetic peptides. Validation includes testing in knockout cell lines or tissues to confirm specificity. Researchers rely on these antibodies to dissect signaling mechanisms, assess drug targeting efficacy, and explore therapeutic strategies modulating NF-κB activity. Their application has advanced understanding of IKKα's dual roles in pro-inflammatory responses and tissue homeostasis.
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