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产品描述：牛铜锌-过氧化物岐化酶(Cu/Zn-SOD)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的SOD1含量。SOD1 即超氧化物歧化酶 1，是机体抗氧化防御系统的关键酶，能催化超氧阴离子发生歧化反应，保护细胞免受氧化应激损伤。在研究中，其与多种神经退行性疾病如渐冻症密切相关，聚焦于基因突变致其功能异常及毒性聚集机制。试剂盒检测范围为15.6 ng/mL-1000 ng/mL，适用于科研领域中对牛机体抗氧化能力评估、氧化应激相关机制研究，例如在营养学、环境毒理学或疾病模型中分析氧化损伤与保护效应。研究人员可通过高效获取血清或血浆样本中Cu/Zn - SOD的定量数据，为探究氧化应激调控网络及抗氧化防御机制提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：SOD1 ELISA Kit; Superoxide dismutase [Cu-Zn] ELISA Kit; EC 1.15.1.1 ELISA Kit
缩写：SOD1
Uniprot No.：P00442
种属：Bos taurus (Bovine)
样本类型：serum, plasma
检测范围：15.6 ng/mL-1000 ng/mL
灵敏度：3.9 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of bovine Cu/Zn-SOD in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 97 | ||||
| Range % | 92-104 | |||||
| 1:2 | Average % | 91 | ||||
| Range % | 85-97 | |||||
| 1:4 | Average % | 90 | ||||
| Range % | 88-95 | |||||
| 1:8 | Average % | 100 | ||||
| Range % | 96-105 | |||||
回收率：
| The recovery of bovine Cu/Zn-SOD spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 87-94 | ||||
| EDTA plasma (n=4) | 101 | 103-105 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected10002.0142.1152.0651.9295001.5371.6401.5891.4532501.2011.1081.1551.0191250.8780.8440.8610.72562.50.5410.5550.5480.41231.20.3420.3290.3360.20015.60.2510.2720.2620.12600.1370.1350.136 | ||||||
货期：3-5 working days
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