| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CASP9; MCH6; Caspase-9; CASP-9; Apoptotic protease Mch-6; Apoptotic protease-activating factor 3; APAF-3; ICE-like apoptotic protease 6; ICE-LAP6 |
| Entrez GeneID | 842 |
| WB Predicted band size | Calculated MW: 46 kDa; Observed MW: 46 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Recombinant protein of human Caspase-9 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
+ +
以下是3篇关于Caspase 9抗体的经典文献摘要信息：
---
1. **文献名称**：*Identification of Caspase-9 Complexed with Apaf-1*
**作者**：Zou, H., Li, Y., Liu, X., Wang, X.
**摘要**：该研究通过免疫共沉淀技术，利用Caspase-9特异性抗体，揭示了Caspase-9与Apaf-1在细胞凋亡信号复合体(apoptosome)中的相互作用机制，证实其在凋亡启动中的核心作用。
2. **文献名称**：*Caspase-9 Deficiency Causes Embryonic Lethality and Defective Apoptosis*
**作者**：Hakem, R., Hakem, A., Duncan, G.S., et al.
**摘要**：通过构建Caspase-9基因敲除小鼠模型，结合抗体检测发现Caspase-9缺失导致胚胎期神经元凋亡受阻，证明其在发育过程中调控线粒体途径凋亡的关键性。
3. **文献名称**：*Ordering the Cytochrome c-Initiated Caspase Cascade*
**作者**：Slee, E.A., Harte, M.T., Kluck, R.M., et al.
**摘要**：研究利用Caspase-9抗体进行Western blot及抑制实验，阐明了Caspase-9在细胞色素c释放后的激活时序及其下游Caspase-3的级联活化路径。
---
以上文献均聚焦于Caspase-9的功能与检测，涵盖结构互作、基因敲除模型及凋亡信号通路机制，抗体在其中主要用于蛋白互作验证、表达水平检测及功能研究。
Caspase-9 antibody is a crucial tool for studying apoptosis, a programmed cell death process essential for development, homeostasis, and disease. Caspase-9. a member of the cysteine-aspartic protease (caspase) family, acts as an initiator caspase in the intrinsic (mitochondrial) apoptotic pathway. It is activated through the apoptosome complex, formed by cytochrome c release, Apaf-1. and ATP, triggering proteolytic cleavage of downstream effector caspases (e.g., caspase-3/7) to execute apoptosis.
Researchers use caspase-9 antibodies to detect its expression, activation, and localization in cells or tissues. These antibodies typically target either the full-length inactive zymogen (procaspase-9. ~46 kDa) or cleaved active forms (~35/37 kDa), aiding in distinguishing between pro-apoptotic and inactive states. Applications include Western blotting, immunohistochemistry, flow cytometry, and immunofluorescence. They are vital in studying apoptosis-related diseases, such as cancer (where caspase-9 dysregulation may promote survival) or neurodegenerative disorders (linked to excessive apoptosis).
Caspase-9 antibodies are often validated across species (human, mouse, rat) and tested for specificity to avoid cross-reactivity with other caspases. Some antibodies recognize phosphorylation sites (e.g., Thr125), providing insights into regulatory mechanisms. Proper controls, like caspase-9 knockout cells, are recommended to confirm signal specificity. Reliable caspase-9 antibodies are indispensable for unraveling apoptotic pathways and evaluating therapeutic strategies targeting cell death regulation.
×
