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产品描述：大鼠超氧化物歧化酶1,可溶性(SOD1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的SOD1含量。SOD1（铜锌超氧化物歧化酶1）是第一个被确定的家族性肌萎缩侧索硬化症（ALS）的遗传基因，其突变会导致蛋白质异常聚集，引发神经元退行。SOD1在细胞内清除超氧自由基，突变后失去功能，导致氧化应激和神经元损伤。研究SOD1有助于理解ALS的发病机制，并开发新的治疗策略。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于体外细胞模型、组织样本或体液中的SOD1定量分析；为研究氧化应激相关疾病机制、药物干预效果评估或生物标志物探索提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Sod1 ELISA Kit; Superoxide dismutase [Cu-Zn] ELISA Kit; EC 1.15.1.1 ELISA Kit
缩写：SOD1
Uniprot No.：P07632
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat SOD1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:200 | Average % | 93 | ||||
| Range % | 88-95 | |||||
| 1:400 | Average % | 104 | ||||
| Range % | 100-107 | |||||
| 1:800 | Average % | 92 | ||||
| Range % | 87-95 | |||||
| 1:1600 | Average % | 97 | ||||
| Range % | 92-100 | |||||
回收率：
| The recovery of rat SOD1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 89-95 | ||||
| EDTA plasma (n=4) | 95 | 92-97 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 10002.7742.7672.7712.638 5002.2262.3052.2662.133 2501.7371.6241.6811.548 1251.1081.0881.0980.965 62.50.6360.6280.6320.499 31.20.3390.3210.3300.197 15.60.2310.2240.2280.095 00.1300.1360.133 | ||||||
货期：3-5 working days
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