首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人锰超氧化物歧化酶(SOD2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、细胞裂解物、组织匀浆样本中的SOD2含量。SOD2（超氧化物歧化酶2）是一种依赖锰的抗氧化酶，主要存在于线粒体中，参与清除细胞内的超氧阴离子自由基，保护细胞免受氧化损伤。研究发现，SOD2在多种疾病中扮演重要角色，如乳腺癌、卵巢癌、宫颈癌等，其表达水平与肿瘤的进展和预后密切相关。研究机制上，SOD2可能通过调节细胞信号通路、影响细胞增殖、侵袭和转移等过程发挥作用。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于体外培养细胞模型中氧化损伤机制研究、抗氧化药物筛选或疾病组织样本中SOD2表达水平分析等科研用途本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Indophenoloxidase B ELISA Kit; IPO B ELISA Kit; IPOB ELISA Kit; Manganese containing superoxide dismutase ELISA Kit; Manganese SOD ELISA Kit; Manganese superoxide dismutase ELISA Kit; Mangano superoxide dismutase ELISA Kit; Mn SOD ELISA Kit; Mn superoxide dismutase ELISA Kit; MNSOD ELISA Kit; MVCD6 ELISA Kit; SOD 2 ELISA Kit; SOD2 ELISA Kit; SODM_HUMAN ELISA Kit; Superoxide dismutase [Mn] mitochondrial ELISA Kit; Superoxide dismutase [Mn], mitochondrial ELISA Kit; Superoxide dismutase 2 mitochondrial ELISA Kit
缩写：SOD2
Uniprot No.：P04179
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates, cell lysates, tissue homogenates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human SOD2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:200 | Average % | 88 | ||||
| Range % | 80-92 | |||||
| 1:400 | Average % | 95 | ||||
| Range % | 91-100 | |||||
| 1:800 | Average % | 100 | ||||
| Range % | 90-110 | |||||
| 1:1600 | Average % | 93 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of human SOD2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 89-98 | ||||
| EDTA plasma (n=4) | 93 | 90-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected10002.2482.1522.2002.1075001.9671.8901.9291.8362501.6851.6041.6451.5521251.3341.3191.3271.23462.50.9250.9040.9150.82231.20.6290.5900.6100.51715.60.2770.2690.2730.18000.0960.0900.093 | ||||||
货期：3-5 working days
×
