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产品描述:人转录激活因子BRG1(SMARCA4)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆、细胞裂解物样本中的SMARCA4含量。SMARCA4是一种重要靶点。它参与染色质重塑过程,对基因表达调控等关键细胞功能意义重大。目前针对SMARCA4的研究,多聚焦于其在肿瘤发生发展中的作用机制,探索以其为靶点开发新的抗癌药物和治疗策略。试剂盒检测范围为25 pg/mL-1600 pg/mL,适用于基础科研中对BRG1蛋白功能的探索,例如肿瘤微环境研究、干细胞分化调控分析或表观遗传学机制解析,支持研究者通过定量检测不同样本中BRG1的动态变化,揭示其在疾病模型或分子通路中的作用机制。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
别名:ATP dependent helicase SMARCA4 ELISA Kit; ATP-dependent helicase SMARCA4 ELISA Kit; BAF 190 ELISA Kit; BAF190 ELISA Kit; BAF190A ELISA Kit; Brahma protein like 1 ELISA Kit; BRG1 ELISA Kit; BRG1 associated factor 190A ELISA Kit; BRG1 protein ELISA Kit; BRG1-associated factor 190A ELISA Kit; BRM/SWI2 related gene 1 ELISA Kit; Global transcription activator homologous sequence ELISA Kit; global transcription activator snf2l4 ELISA Kit; Homeotic gene regulator ELISA Kit; hSNF2b ELISA Kit; Mitotic growth and transcription activator ELISA Kit; MRD16 ELISA Kit; Nuclear protein GRB1 ELISA Kit; Protein brahma homolog 1 ELISA Kit; Protein BRG-1 ELISA Kit; Protein BRG1 ELISA Kit; RTPS2 ELISA Kit; SMARC A4 ELISA Kit; SMARCA4 ELISA Kit; SMCA4_HUMAN ELISA Kit; SNF2 ELISA Kit; SNF2 beta ELISA Kit; SNF2 like 4 ELISA Kit; SNF2-beta ELISA Kit; SNF2B ELISA Kit; SNF2L4 ELISA Kit; SNF2LB ELISA Kit; Sucrose nonfermenting like 4 ELISA Kit; SWI/SNF related matrix associated actin dependent regulator of chromatin subfamily A member 4 ELISA Kit; SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 ELISA Kit; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 4 ELISA Kit; SWI2 ELISA Kit; Transcription activator BRG1 ELISA Kit
缩写:SMARCA4
Uniprot No.:P51532
种属:Homo sapiens (Human)
样本类型:serum, plasma, tissue homogenates, cell lysates
检测范围:25 pg/mL-1600 pg/mL
灵敏度:6.25 pg/mL
反应时间:1-5h
样本体积:50-100ul
检测波长:450 nm
研究领域:Neuroscience
测定原理:quantitative
测定方法:Sandwich
精密度:
Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
Inter-assay Precision (Precision between assays): CV%<10% | |||||||
Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human SMARCA4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
Sample | Serum(n=4) | ||||||
1:1 | Average % | 87 | |||||
Range % | 82-91 | ||||||
1:2 | Average % | 104 | |||||
Range % | 98-107 | ||||||
1:4 | Average % | 94 | |||||
Range % | 88-98 | ||||||
1:8 | Average % | 92 | |||||
Range % | 88-96 |
回收率:
The recovery of human SMARCA4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
Sample Type | Average % Recovery | Range | |||||
Serum (n=5) | 91 | 86-95 | |||||
EDTA plasma (n=4) | 89 | 87-92 | |||||
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
pg/mlOD1OD2AverageCorrected16002.9652.9092.9372.7638002.3422.2152.2792.1054001.4951.4621.4791.3052000.9140.9000.9070.7331000.5670.5550.5610.387500.3780.3630.3710.197250.2480.2430.2460.07200.1750.1730.174 |
货期:3-5 working days
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