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产品描述：人母亲DPP同源物4(Smad 4)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的SMAD4含量。SMAD4是重要的信号转导分子，在TGF - β信号通路中起关键作用。其背景上与肿瘤等多种疾病的发生发展密切相关。研究机制方面，它参与调控细胞生长、分化、凋亡等过程，功能异常会打破细胞稳态，引发疾病，是研究疾病机理和治疗靶点的热点。试剂盒检测范围为25 pg/mL-1600 pg/mL，在科研领域，本试剂盒可广泛应用于TGF-β信号转导机制研究、肿瘤微环境分析、基因功能验证等方向，特别适用于探索Smad4在胚胎发育、组织修复及疾病模型中的作用机制，为分子生物学、细胞生物学及病理学相关研究提供可靠的数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：(Small) Mothers Against Decapentaplegic ELISA Kit; Deleted in Pancreatic Carcinoma 4 ELISA Kit; Deleted in Pancreatic Carcinoma ELISA Kit; Deleted in pancreatic carcinoma locus 4 ELISA Kit; Deletion target in pancreatic carcinoma 4 ELISA Kit; DPC 4 ELISA Kit; DPC4 ELISA Kit; hSMAD4 ELISA Kit; JIP ELISA Kit; MAD homolog 4 ELISA Kit; MAD mothers against decapentaplegic Drosophila homolog 4 ELISA Kit; MAD mothers against decapentaplegic homolog 4 ELISA Kit; MADH 4 ELISA Kit; MADH4 ELISA Kit; Med ELISA Kit; Medea ELISA Kit; Mothers against decapentaplegic homolog 4 ELISA Kit; Mothers against decapentaplegic; Drosophila; homolog of; 4 ELISA Kit; Mothers against DPP homolog 4 ELISA Kit; MYHRS ELISA Kit; OTTHUMP00000163548 ELISA Kit; SMA- and MAD-related protein 4 ELISA Kit; SMAD 4 ELISA Kit; SMAD family member 4 ELISA Kit; SMAD mothers against DPP homolog 4 ELISA Kit; SMAD4 ELISA Kit; SMAD4_HUMAN ELISA Kit
缩写：SMAD4
Uniprot No.：Q13485
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：25 pg/mL-1600 pg/mL
灵敏度：6.25 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Epigenetics and Nuclear Signaling
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human Smad4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 93 | ||||
| Range % | 89-101 | |||||
| 1:2 | Average % | 92 | ||||
| Range % | 88-100 | |||||
| 1:4 | Average % | 90 | ||||
| Range % | 82-96 | |||||
| 1:8 | Average % | 89 | ||||
| Range % | 82-103 | |||||
回收率：
| The recovery of human Smad4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 88-96 | ||||
| EDTA plasma (n=4) | 95 | 88-105 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected16002.6372.5432.5902.4528001.8231.7681.7961.6584000.9570.9350.9460.8082000.5490.5670.5580.4201000.3520.3620.3570.219500.2190.2270.2230.085250.1780.1750.1770.03900.1390.1370.138 | ||||||
货期：3-5 working days
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