| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | GMA; S-MAG; SIGLEC4A; SIGLEC-4A |
| WB Predicted band size | 69 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human MAG |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇与Caveolin 1抗体相关的代表性文献：
1. **文献名称**: "Cell-type specific expression of caveolin-1 in the lung"
**作者**: Scherer, P.E., et al.
**摘要**: 该研究利用Caveolin 1特异性抗体，通过免疫组化和Western blot分析，揭示了Caveolin 1在不同肺细胞类型(如内皮细胞和肺泡上皮细胞)中的差异表达，证实了其在细胞膜微结构域(如脂筏)中的定位。
2. **文献名称**: "Caveolin-1 null mice are viable but show endothelial cell abnormalities"
**作者**: Razani, B., et al.
**摘要**: 通过基因敲除小鼠模型，结合Caveolin 1抗体的免疫荧光和免疫印迹技术，研究发现Caveolin 1缺失导致内皮细胞功能障碍，揭示了其在血管生成和信号转导中的关键作用。
3. **文献名称**: "Caveolae: from cell biology to animal physiology"
**作者**: Parton, R.G.
**摘要**: 这篇综述总结了Caveolin 1抗体的应用，包括其在超微结构定位(如电子显微镜)和功能研究中的重要性，强调了抗体在解析Caveolae介导的内吞和胆固醇运输机制中的价值。
4. **文献名称**: "Role of caveolin-1 in the pathogenesis of fibrosis in kidney disease"
**作者**: Cohen, A.W., et al.
**摘要**: 研究使用Caveolin 1抗体检测肾脏纤维化模型中该蛋白的表达变化，发现其下调与TGF-β信号通路激活相关，提示Caveolin 1在抑制纤维化中的潜在功能。
以上文献涵盖了Caveolin 1抗体在基础研究、疾病模型及方法学中的应用。
Caveolin-1 (CAV1) is a critical structural protein of caveolae, small plasma membrane invaginations involved in vesicular trafficking, signal transduction, and lipid homeostasis. It plays essential roles in regulating cellular processes such as endocytosis, cholesterol transport, and mechanoprotection. CAV1 interacts with signaling molecules (e.g., Src kinases, G-proteins) and receptors (e.g., EGFR, TGF-βR), modulating pathways like MAPK/ERK and PI3K/AKT. Dysregulation of CAV1 is linked to cancer progression, cardiovascular diseases, fibrosis, and metabolic disorders.
Antibodies targeting CAV1 are vital tools for studying its expression, localization, and function. They are widely used in techniques like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and co-immunoprecipitation (Co-IP). Specific applications include assessing CAV1 downregulation in tumors (e.g., breast, prostate cancers) or its upregulation in fibrotic tissues and atherosclerotic plaques. Commercial CAV1 antibodies are often raised against epitopes in the N-terminal (amino acids 1-178) or C-terminal regions, with clones like 7C8. D46G3. and E249 being common.
Researchers should validate antibodies for target species (human, mouse, rat) and consider tissue-specific isoforms or post-translational modifications (e.g., phosphorylation at Tyr14). Proper controls (e.g., knockout cell lines) are essential due to potential cross-reactivity with Caveolin-2 or non-specific bands. Understanding CAV1's dual roles (tumor-suppressive or oncogenic, depending on context) highlights the importance of precise antibody characterization in experimental design.
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