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产品描述：人Slit同源蛋白3(SLIT3)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液样本中的SLIT3含量。试剂盒检测范围为0.156 ng/mL-10 ng/mL，科研人员可将其应用于体外细胞模型中SLIT3分泌动态监测、疾病动物模型血清标志物研究，或探索特定病理状态下SLIT3与细胞信号通路的关联机制，为神经生物学、肿瘤学及血管相关疾病的基础研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：FLJ10764 ELISA Kit; KIAA0814 ELISA Kit; MEGF 5 ELISA Kit; MEGF5 ELISA Kit; Multiple EGF-like domains protein 5 ELISA Kit; Multiple epidermal growth factor like domains 5 ELISA Kit; Multiple epidermal growth factor-like domains protein 5 ELISA Kit; SLIL 2 ELISA Kit; SLIL2 ELISA Kit; SLIT 1 ELISA Kit; Slit 2 ELISA Kit; Slit 3 ELISA Kit; Slit homolog 3 (Drosophila) ELISA Kit; Slit homolog 3 ELISA Kit; Slit homolog 3 protein ELISA Kit; Slit-3 ELISA Kit; SLIT1 ELISA Kit; Slit2 ELISA Kit; Slit3 ELISA Kit; SLIT3_HUMAN ELISA Kit
缩写：SLIT3
Uniprot No.：O75094
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human SLIT3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 93 | |||||
| Range % | 90-97 | ||||||
| 1:2 | Average % | 85 | |||||
| Range % | 82-93 | ||||||
| 1:4 | Average % | 110 | |||||
| Range % | 105-115 | ||||||
| 1:8 | Average % | 106 | |||||
| Range % | 102-117 | ||||||
回收率：
| The recovery of human SLIT3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 90 | 86-95 | |||||
| EDTA plasma (n=4) | 99 | 95-102 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected 101.8661.9551.9111.777 51.4721.5881.5301.396 2.51.1621.2011.1821.048 1.250.8640.8730.8690.735 0.6250.5250.5580.5420.408 0.3120.3120.3130.3130.179 0.1560.2670.2760.2720.138 00.1370.1310.134 | |||||||
货期：3-5 working days
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