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产品描述:人分泌性Ly-6/uPAR相关蛋白1(SLURP1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆样本中的SLURP1含量。试剂盒检测范围为4.68 ng/mL-300 ng/mL,适用于体外检测科研场景,可支持研究者分析SLURP1在不同生理或病理条件下的表达水平变化,为皮肤病学、肿瘤微环境研究、免疫调节机制探索等基础研究提供量化工具。可满足细胞实验、动物模型或人体样本中SLURP1蛋白水平的定量分析需求。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
别名:SLURP1 ELISA Kit; ARS ELISA Kit; Secreted Ly-6/uPAR-related protein 1 ELISA Kit; SLURP-1 ELISA Kit; ARS component B ELISA Kit; ARS(component B)-81/S ELISA Kit; Anti-neoplastic urinary protein ELISA Kit; ANUP ELISA Kit
缩写:SLURP1
Uniprot No.:P55000
种属:Homo sapiens (Human)
样本类型:serum, plasma, tissue homogenates
检测范围:4.68 ng/mL-300 ng/mL
灵敏度:1.17 ng/mL
反应时间:1-5h
样本体积:50-100ul
检测波长:450 nm
研究领域:Cardiovascular
测定原理:quantitative
测定方法:Sandwich
精密度:
Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<10% | ||||||
Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human SLURP1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
Sample | Serum(n=4) | |||||
1:1 | Average % | 84 | ||||
Range % | 80-92 | |||||
1:2 | Average % | 97 | ||||
Range % | 91-105 | |||||
1:4 | Average % | 100 | ||||
Range % | 96-106 | |||||
1:8 | Average % | 95 | ||||
Range % | 88-100 |
回收率:
The recovery of human SLURP1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 90 | 88-94 | ||||
EDTA plasma (n=4) | 87 | 82-96 | ||||
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
ng/mlOD1OD2AverageCorrected3002.4502.6132.5322.4451502.1212.1692.1452.058751.6761.6871.6821.59537.51.2341.2691.2521.16518.750.6770.6960.6870.6009.3750.4110.4250.4180.3314.690.2370.2460.2420.15500.0870.0860.087 |
货期:3-5 working days
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