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产品描述：人去乙酰化酶Sirtuin-6 (SIRT6/SIR2L6)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的SIRT6含量。SIRT6是一种重要靶点。它属于沉默调节蛋白家族成员，在DNA修复、代谢调控、衰老等生理过程中起关键作用。研究机制上，其通过去乙酰化等修饰特定底物蛋白，参与多条信号通路，影响细胞功能与机体生理状态，是科研关注焦点。试剂盒检测范围为3.12 pg/mL-200 pg/mL，适用于体外实验环境，可为细胞模型、动物模型或人类样本中的SIRT6蛋白表达分析提供稳定工具，支持分子机制研究、药物筛选评估或生物标志物探索等科研需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：2810449N18Rik ELISA Kit; AI043036 ELISA Kit; Mono ADP ribosyltransferase sirtuin 6 ELISA Kit; NAD-dependent protein deacetylase sirtuin-6 ELISA Kit; Regulatory protein SIR2 homolog 6 ELISA Kit; Regulatory protein SIR2 homolog ELISA Kit; SIR2 like 6 ELISA Kit; SIR2 like protein 6 ELISA Kit; Sir2 related protein type 6 ELISA Kit; SIR2-like protein 6 ELISA Kit; SIR2L6 ELISA Kit; SIR6_HUMAN ELISA Kit; SIRT 6 ELISA Kit; SIRT6 ELISA Kit; Sirtuin (silent mating type information regulation 2 homolog) 6 (S. cerevisiae) ELISA Kit; Sirtuin 6 ELISA Kit; Sirtuin type 6 ELISA Kit; Sirtuin6 ELISA Kit
缩写：SIRT6
Uniprot No.：Q8N6T7
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：3.12 pg/mL-200 pg/mL
灵敏度：0.78 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Epigenetics and Nuclear Signaling
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human SIRT6/SIR2L6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 99 | ||||
| Range % | 94-104 | |||||
| 1:200 | Average % | 103 | ||||
| Range % | 99-107 | |||||
| 1:400 | Average % | 89 | ||||
| Range % | 85-95 | |||||
| 1:800 | Average % | 96 | ||||
| Range % | 93-99 | |||||
回收率：
| The recovery of human SIRT6/SIR2L6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 88-98 | ||||
| EDTA plasma (n=4) | 103 | 99-107 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected2002.5682.6892.6292.5121001.8351.9651.9001.783501.3891.3211.3551.238250.8660.8420.8540.73712.50.5740.5350.5550.4386.250.3220.3350.3290.2123.120.2090.2040.2070.09000.1170.1160.117 | ||||||
货期：3-5 working days
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