首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠NAD依赖性蛋白脱乙酰酶sirtuin-1(SIRT1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的SIRT1含量。SIRT1 是一种与细胞代谢、衰老及应激反应等密切相关的靶点。其研究机制主要围绕其作为组蛋白去乙酰化酶的功能，它能通过去乙酰化作用调控多种底物，参与能量代谢、基因表达调控等生物学过程，在衰老相关疾病等研究领域备受关注。试剂盒检测范围为0.156 ng/mL-10 ng/mL，适用于体外培养细胞、动物模型组织等科研样本的蛋白定量分析；通过检测SIRT1在代谢相关疾病模型中的表达动态，可深入探索其在胰岛素敏感性调节、线粒体功能维护以及炎症反应调控中的分子机制，为抗衰老研究和代谢综合征机制解析提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Sirt1 ELISA Kit; Sir2l1 ELISA Kit; NAD-dependent protein deacetylase sirtuin-1 ELISA Kit; EC 2.3.1.286 ELISA Kit; NAD-dependent protein deacylase sirtuin-1 ELISA Kit; EC 2.3.1.- ELISA Kit; Regulatory protein SIR2 homolog 1 ELISA Kit; SIR2-like protein 1 ELISA Kit; SIR2alpha ELISA Kit; Sir2 ELISA Kit; mSIR2a) [Cleaved into: SirtT1 75 kDa fragment ELISA Kit; 75SirT1)] ELISA Kit
缩写：SIRT1
Uniprot No.：Q923E4
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Epigenetics and Nuclear Signaling
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse Sirt1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 90 | ||||
| Range % | 85-95 | |||||
| 1:2 | Average % | 93 | ||||
| Range % | 88-98 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 89-100 | |||||
| 1:8 | Average % | 97 | ||||
| Range % | 93-103 | |||||
回收率：
| The recovery of mouse Sirt1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 99 | 95-104 | ||||
| EDTA plasma (n=4) | 98 | 92-104 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 102.3222.1622.2422.071 51.5161.4471.4821.311 2.50.8970.8340.8660.695 1.250.5120.5090.5110.340 0.6250.3490.3270.3380.167 0.3120.2780.2580.2680.097 0.1560.2320.2190.2260.055 00.1750.1670.171 | ||||||
货期：3-5 working days
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