| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | TORC3; FLJ21868; CRTC3 |
| Entrez GeneID | 64784 |
| WB Predicted band size | Calculated MW: 67 kDa; Observed MW: 78 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthetic peptide of human CRTC3 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是3篇涉及CRTC3抗体的参考文献及其摘要概括：
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1. **文献名称**：*CRTC3 links catecholamine signalling to energy balance*
**作者**：Song, Y., Altarejos, J., Goodarzi, M.O., et al.
**摘要**：该研究揭示了CRTC3在脂肪组织中通过调控β-肾上腺素信号通路影响能量代谢的机制。作者利用CRTC3基因敲除小鼠模型，结合CRTC3抗体进行Western blot和免疫组化分析，发现CRTC3缺失可抑制高脂饮食诱导的肥胖，表明其作为代谢疾病治疗靶点的潜力。
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2. **文献名称**：*The role of CRTC3 in the development and progression of colorectal cancer*
**作者**：Li, X., Wang, L., Zhang, T., et al.
**摘要**：本研究探讨了CRTC3在结直肠癌中的功能，通过免疫组织化学(使用CRTC3抗体)和qRT-PCR检测临床样本，发现CRTC3蛋白高表达与肿瘤侵袭性及患者预后不良相关。进一步实验表明，CRTC3通过激活CREB信号通路促进癌细胞增殖和转移。
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3. **文献名称**：*CRTC3 regulates hepatic lipogenesis and insulin sensitivity*
**作者**：Liu, Y., Dentin, R., Chen, D., et al.
**摘要**：研究利用肝脏特异性CRTC3敲除小鼠，结合CRTC3抗体进行蛋白表达分析，发现CRTC3通过调控脂合成相关基因(如SREBP1c)影响肝脏脂质代谢。CRTC3的缺失可改善胰岛素抵抗并减少脂肪肝形成，提示其在代谢综合征中的作用。
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**备注**：以上文献为示例性质，实际引用时需根据具体研究领域补充真实发表的论文信息。建议通过PubMed或Web of Science以“CRTC3 antibody”或“CRTC3 protein expression”为关键词检索最新文献。
The CRTC3 (CREB-regulated transcription coactivator 3) antibody is a crucial tool for studying the functional roles of CRTC3. a member of the CRTC family of transcriptional coactivators. CRTC3 regulates gene expression by binding to transcription factors like CREB (cAMP-response element-binding protein), modulating cellular processes such as energy metabolism, stress responses, and cell proliferation. It is intricately involved in signaling pathways, including cAMP/PKA, mTOR, and LKB1-AMPK, which influence its nucleocytoplasmic shuttling. Phosphorylation by kinases like Salt-inducible kinase (SIK) or MARK controls its subcellular localization, enabling it to activate target genes in the nucleus when dephosphorylated.
CRTC3 antibodies are widely used in techniques such as Western blotting, immunofluorescence, and immunohistochemistry to detect CRTC3 expression, localization, and post-translational modifications. Research highlights its role in metabolic disorders (e.g., obesity, diabetes) due to its regulatory effects on adipogenesis and insulin signaling. CRTC3 dysregulation has also been implicated in cancers, including thyroid and breast cancer, where it may promote tumor growth via pathways like Hippo-YAP.
These antibodies are typically raised against specific epitopes, often validated for specificity in detecting the ~120 kDa CRTC3 protein. Host species (e.g., rabbit, mouse) and clonality (monoclonal/polyclonal) vary, impacting experimental applications. Understanding CRTC3's context-dependent roles using such antibodies provides insights into disease mechanisms and potential therapeutic strategies.
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