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Human Sialic acid-binding Ig-like lectin 9(SIGLEC9) ELISA kit

  • 中文名： 人唾液酸结合Ig样凝集素9(SIGLEC9)酶联免疫试剂盒
货号: IPD-EL021303HU
Price： ￥2500
数量：
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产品详情

  • 产品描述：
    人唾液酸结合Ig样凝集素9(SIGLEC9)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆，组织匀浆.样本中的SIGLEC9含量。SIGLEC9是唾液酸结合免疫球蛋白样凝集素家族成员，广泛分布于免疫细胞表面。它能识别细胞表面糖基配体，参与免疫信号传导，调控免疫细胞活性和功能。当前围绕其展开多项研究，旨在明晰机制，为免疫相关疾病治疗提供潜在靶点。试剂盒检测范围为125 pg/ml-8000 pg/ml，适用于科研场景中探究SIGLEC9在免疫应答、疾病模型或病理状态下的动态变化，例如通过检测不同处理条件下细胞培养上清、实验动物体液或疾病相关组织样本中的蛋白浓度，为免疫调控机制、生物标志物筛选等基础研究提供可靠数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    CD329 ELISA Kit; CDw329 ELISA Kit; FOAP 9 ELISA Kit; OB binding protein like ELISA Kit; OBBP LIKE ELISA Kit; Protein FOAP 9 ELISA Kit; Protein FOAP-9 ELISA Kit; sialic acid binding Ig like lectin 9 ELISA Kit; Sialic acid-binding Ig-like lectin 9 ELISA Kit; Sialic acid-binding immunoglobulin like lectin 9 ELISA Kit; SIGL9_HUMAN ELISA Kit; Siglec 9 ELISA Kit; Siglec-9 ELISA Kit; SIGLEC9 ELISA Kit
  • 缩写：
    SIGLEC9
  • Uniprot No.：
    Q9Y336
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum,plasma,tissue homogenates.
  • 检测范围：
    125 pg/ml-8000 pg/ml
  • 灵敏度：
    31.25 pg/ml
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human SIGLEC9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    95


    Range %

    88-102


    1:2

    Average %

    87


    Range %

    83-92


    1:4

    Average %

    92


    Range %

    88-97


    1:8

    Average %

    89


    Range %

    85-95


  • 回收率：

    The recovery of human SIGLEC9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    90

    85-96


    EDTA plasma (n=4)

    96

    90-102


  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    pg/ml

    OD1

    OD2

    Average

    Corrected


    8000

    2.169

    2.234

    2.202

    2.068


    4000

    1.378

    1.297

    1.338

    1.205


    2000

    0.831

    0.828

    0.829

    0.696


    1000

    0.474

    0.489

    0.481

    0.348


    500

    0.383

    0.361

    0.372

    0.239


    250

    0.250

    0.253

    0.251

    0.118


    125

    0.186

    0.184

    0.185

    0.052


    0

    0.137

    0.129

    0.133




  • 货期：
    3-5 working days

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