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产品描述：人酪氨酸-蛋白磷酸酶非受体型底物1(SIRPA)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的SIRPA含量。SIRPA，即信号调节蛋白α，是一种跨膜蛋白，主要在髓系细胞表面表达。其通过与CD47结合，传递抑制性信号，抑制巨噬细胞吞噬作用，参与免疫调节。SIRPA在肿瘤免疫逃逸中发挥重要作用，成为肿瘤免疫治疗的潜在靶点。试剂盒检测范围为0.156 ng/mL-10 ng/mL，本试剂盒适用于基础科研领域中对SIRPA蛋白表达调控、信号通路互作及相关疾病模型的体外研究，为探索SIRPA在免疫微环境中的作用提供高效工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Signal regulatory protein alpha type 1 ELISA Kit; Bit ELISA Kit; Brain Ig like molecule with tyrosine based activation motifs ELISA Kit; Brain Ig-like molecule with tyrosine-based activation motifs ELISA Kit; Brain immunoglobulin like molecule with tyrosine based activation motifs ELISA Kit; CD172 antigen like family member A ELISA Kit; CD172 antigen-like family member A ELISA Kit; CD172a ELISA Kit; CD172a antigen ELISA Kit; Inhibitory receptor SHPS-1 ELISA Kit; Macrophage fusion receptor ELISA Kit; MFR ELISA Kit; MYD 1 ELISA Kit; Myd 1 antigen ELISA Kit; MyD-1 antigen ELISA Kit; p84 ELISA Kit; Protein tyrosine phosphatase non receptor type substrate 1 ELISA Kit; PTPNS1 ELISA Kit; SHP substrate 1 ELISA Kit; SHPS-1 ELISA Kit; SHPS1 ELISA Kit; SHPS1_HUMAN ELISA Kit; Signal regulatory protein alpha 2 ELISA Kit; Signal regulatory protein alpha 3 ELISA Kit; Signal regulatory protein alpha ELISA Kit; Signal regulatory protein alpha type 2 ELISA Kit; Signal-regulatory protein alpha-1 ELISA Kit; Signal-regulatory protein alpha-2 ELISA Kit; Signal-regulatory protein alpha-3 ELISA Kit; SIRP ELISA Kit; Sirp-alpha-1 ELISA Kit; Sirp-alpha-2 ELISA Kit; Sirp-alpha-3 ELISA Kit; SIRPA ELISA Kit; SIRPalpha ELISA Kit; SIRPalpha1 ELISA Kit; SIRPalpha2 ELISA Kit; SIRPalpha3 ELISA Kit; Tyrosine phosphatase SHP substrate 1 ELISA Kit; Tyrosine protein phosphatase non receptor type substrate 1 ELISA Kit; Tyrosine-protein phosphatase non-receptor type substrate 1 ELISA Kit
缩写：SIRPA
Uniprot No.：P78324
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human SIRPA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 90 | ||||
| Range % | 85-96 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 91-105 | |||||
| 1:4 | Average % | 98 | ||||
| Range % | 95-106 | |||||
| 1:8 | Average % | 86 | ||||
| Range % | 80-92 | |||||
回收率：
| The recovery of human SIRPA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 89 | 86-96 | ||||
| EDTA plasma (n=4) | 92 | 88-97 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected102.2282.1282.1782.06451.5271.4581.4931.3792.50.8680.8380.8530.7391.250.4270.4170.4220.3080.6250.2980.2880.2930.1790.3120.2200.2170.2190.1050.1560.1740.1700.1720.05800.1150.1120.114 | ||||||
货期：3-5 working days
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