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产品描述：人NAD依赖性蛋白去乙酰化酶sirtuin-3,线粒体(SIRT3)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的SIRT3含量。SIRT3是重要靶点。它属于Ⅲ类组蛋白去乙酰化酶，在线粒体中发挥关键作用。研究机制上，其通过去乙酰化修饰调控线粒体蛋白功能，参与能量代谢、氧化应激等过程，与衰老、代谢疾病、肿瘤等多种病症的发生发展密切相关。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于研究SIRT3在代谢综合征、神经退行性疾病、肿瘤微环境调控等领域的分子机制。特别适用于科研人员通过血清/血浆样本分析循环系统中SIRT3的动态变化，或利用组织匀浆探究特定器官中线粒体功能的分子调控网络。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：hSIRT 3 ELISA Kit; hSIRT3 ELISA Kit; Mitochondrial nicotinamide adenine dinucleotide dependent deacetylase ELISA Kit; NAD dependent deacetylase sirtuin 3 mitochondrial ELISA Kit; NAD-dependent protein deacetylase sirtuin-3, mitochondrial ELISA Kit; Regulatory protein SIR2 homolog 3 ELISA Kit; Silent mating type information regulation 2 S.cerevisiae homolog 3 ELISA Kit; Sir 2 like 3 ELISA Kit; SIR 2 like protein 3 ELISA Kit; SIR 3 ELISA Kit; SIR2 L3 ELISA Kit; Sir2 like 3 ELISA Kit; SIR2 like protein 3 ELISA Kit; SIR2-like protein 3 ELISA Kit; SIR2L3 ELISA Kit; SIR3_HUMAN ELISA Kit; SIRT 3 ELISA Kit; SIRT3 ELISA Kit; Sirtuin 3 ELISA Kit; Sirtuin silent mating type information regulation 2 homolog 3 (S. cerevisiae) ELISA Kit; Sirtuin type 3 ELISA Kit; Sirtuin3 ELISA Kit
缩写：SIRT3
Uniprot No.：Q9NTG7
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Epigenetics and Nuclear Signaling
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human SIRT3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 89 | ||||
| Range % | 82-95 | |||||
| 1:2 | Average % | 92 | ||||
| Range % | 86-99 | |||||
| 1:4 | Average % | 98 | ||||
| Range % | 90-105 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 88-100 | |||||
回收率：
| The recovery of human SIRT3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 90-103 | ||||
| EDTA plasma (n=4) | 90 | 84-97 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected10002.7432.6122.6782.5405002.3122.1562.2342.0962501.7131.6861.7001.5621251.0211.0091.0150.87762.50.5140.5570.5360.39831.20.3210.3010.3110.17315.60.2030.1960.2000.06200.1390.1370.138 | ||||||
货期：3-5 working days
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