首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人皮质类固醇结合球蛋白( CBG )酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的SERPINA6含量。试剂盒检测范围为20 pmol/mL-8000 pmol/mL，适用于探索CBG在内分泌调控、应激反应机制、肝脏功能及相关疾病模型中的表达变化，或用于评估药物干预对激素运输蛋白的影响研究。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CBG ELISA Kit; CBG_HUMAN ELISA Kit; corticosteroid binding globulin ELISA Kit; Corticosteroid-binding globulin ELISA Kit; serine (or cysteine) proteinase inhibitor clade A (alpha 1 antiproteinase antitrypsin) member 6 ELISA Kit; Serpin A6 ELISA Kit; serpin peptidase inhibitor clade A (alpha 1 antiproteinase antitrypsin) member 6 ELISA Kit; Serpina6 ELISA Kit; Transcortin ELISA Kit
缩写：SERPINA6
Uniprot No.：P08185
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：20 pmol/mL-8000 pmol/mL
灵敏度：20 pmol/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human CBG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 95 | ||||
| Range % | 91-100 | |||||
| 1:2 | Average % | 93 | ||||
| Range % | 88-99 | |||||
| 1:4 | Average % | 93 | ||||
| Range % | 89-100 | |||||
| 1:8 | Average % | 97 | ||||
| Range % | 91-108 | |||||
回收率：
| The recovery of human CBG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 92-99 | ||||
| EDTA plasma (n=4) | 92 | 89-95 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pmol/mlOD1OD2Average80000.4110.4070.40915000.7720.7650.7692501.2091.1981.204701.5881.5841.586201.7461.7361.74102.4622.4662.464 | ||||||
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