首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠内脏脂肪特异性丝氨酸蛋白酶抑制剂(vaspin)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的SERPINA12含量。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，可广泛应用于肥胖动物模型建立、代谢性疾病机制研究、脂肪组织功能评估以及药物干预效果评价等科研领域，为探索vaspin在能量代谢调控中的分子机制提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Serpina12 ELISA Kit; Serpin A12 ELISA Kit; Visceral adipose tissue-derived serine protease inhibitor ELISA Kit; Vaspin ELISA Kit; Visceral adipose-specific serpin ELISA Kit
缩写：SERPINA12
Uniprot No.：Q8R4Z1
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat vaspin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 85 | ||||
| Range % | 80-90 | |||||
| 1:2 | Average % | 94 | ||||
| Range % | 90-98 | |||||
| 1:4 | Average % | 91 | ||||
| Range % | 85-97 | |||||
| 1:8 | Average % | 97 | ||||
| Range % | 92-104 | |||||
回收率：
| The recovery of rat vaspin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 105 | 100-110 | ||||
| EDTA plasma (n=4) | 95 | 90-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected20002.6792.5762.6282.47910002.0712.1142.0931.9445001.5821.4721.5271.3782500.9580.9130.9360.7871250.5800.5890.5850.43662.50.4120.4070.4100.26131.250.3020.2790.2910.14200.1470.1510.149 | ||||||
货期：3-5 working days
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