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产品描述：人血浆丝氨酸蛋白酶抑制剂(SERPINA5)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、组织培养上清液、尿液样本中的SERPINA5含量。SERPINA5属于丝氨酸蛋白酶抑制剂家族。它在调节体内蛋白酶活性、维持生理平衡等方面有重要作用。研究机制上，其主要通过与特定蛋白酶结合抑制其活性。相关研究有助于了解疾病发病机制，为药物研发尤其是针对蛋白酶失衡疾病提供新靶点。试剂盒检测范围为0.156 ng/mL-10 ng/mL，该产品适用于体外研究SERPINA5在凝血异常、炎症性疾病或肿瘤进展中的分子机制，也可用于筛选调节SERPINA5表达的药物或评估其在特定病理模型中的动态变化，为生物标志物探索及分子互作研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Acrosomal serine protease inhibitor ELISA Kit; IPSP ELISA Kit; IPSP_HUMAN ELISA Kit; PAI 3 ELISA Kit; PAI-3 ELISA Kit; PAI3 ELISA Kit; PCI ELISA Kit; PCI-B ELISA Kit; PLANH 3 ELISA Kit; PLANH3 ELISA Kit; Plasma serine protease inhibitor ELISA Kit; Plasminogen activator inhibitor 3 ELISA Kit; Plasminogen activator inhibitor III ELISA Kit; PROCI ELISA Kit; Protein C inhibitor ELISA Kit; Serpin A5 ELISA Kit; Serpin peptidase inhibitor clade A (alpha 1 antiproteinase antitrypsin) member 5 ELISA Kit; Serpin peptidase inhibitor clade A member 5 ELISA Kit; Serpina5 ELISA Kit
缩写：SERPINA5
Uniprot No.：P05154
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell culture supernates, urine
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human SERPINA5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:200 | Average % | 97 | ||||
| Range % | 92-102 | |||||
| 1:400 | Average % | 91 | ||||
| Range % | 82-96 | |||||
| 1:800 | Average % | 103 | ||||
| Range % | 98-109 | |||||
| 1:1600 | Average % | 87 | ||||
| Range % | 82-96 | |||||
回收率：
| The recovery of human SERPINA5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 85-96 | ||||
| EDTA plasma (n=4) | 87 | 84-92 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected102.8892.7582.8242.69452.3882.3872.3882.2582.52.0201.9051.9631.8331.251.4801.5081.4941.3640.6250.9200.9530.9370.8070.3120.4650.4740.4700.3400.1560.2420.2510.2470.11700.1320.1270.130 | ||||||
货期：3-5 working days
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