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Human Proinsulin,PI ELISA Kit

  • 中文名： 人胰岛素原(PI)ELISA Kit
货号: IPD-E08345h
Price： ￥2500
数量：
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产品详情

  • 产品描述：
    人胰岛素原(PI)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆,组织匀浆样本中的PI含量。试剂盒检测范围为62.5 pg/mL-4000 pg/mL，适用于探索2型糖尿病发病机制、胰岛移植效果评估、胰岛素抵抗研究及药物干预对β细胞功能的影响等科研场景。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    N/A
  • 缩写：
    PI
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma,tissue homogenates
  • 检测范围：
    62.5 pg/mL-4000 pg/mL
  • 灵敏度：
    15.6 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human PI in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    91


    Range %

    87-97


    1:2

    Average %

    99


    Range %

    96-104


    1:4

    Average %

    101


    Range %

    95-106


    1:8

    Average %

    95


    Range %

    90-98


  • 回收率：

    The recovery of human PI spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    91

    87-97


    EDTA plasma (n=4)

    99

    93-105


  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    pg/ml

    OD1

    OD2

    Average

    Corrected


    4000

    2.255

    2.187

    2.221

    2.083


    2000

    1.638

    1.732

    1.685

    1.547


    1000

    1.215

    1.314

    1.265

    1.127


    500

    0.788

    0.802

    0.795

    0.657


    250

    0.462

    0.501

    0.482

    0.344


    125

    0.355

    0.346

    0.351

    0.213


    62.5

    0.247

    0.238

    0.243

    0.105


    0

    0.139

    0.137

    0.138




  • 货期：
    3-5 working days

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