首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人抑制素(PHB)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的PHB含量。试剂盒检测范围为23.5 pg/mL-1500 pg/mL，该产品适用于科研领域PHB相关研究，例如肿瘤微环境分析、代谢通路调控机制探索、细胞衰老模型构建等基础研究，通过检测不同样本中PHB的表达差异，为疾病分子机制解析及药物干预效果评估提供实验依据。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Epididymis luminal protein 215 ELISA Kit; Epididymis secretory sperm binding protein Li 54e ELISA Kit; HEL 215 ELISA Kit; HEL S 54e ELISA Kit; PHB ELISA Kit; PHB_HUMAN ELISA Kit; PHB1 ELISA Kit; Prohibitin ELISA Kit
缩写：PHB
Uniprot No.：P35232
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：23.5 pg/mL-1500 pg/mL
灵敏度：5.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human PHB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 84 | ||||
| Range % | 80-92 | |||||
| 1:2 | Average % | 100 | ||||
| Range % | 95-108 | |||||
| 1:4 | Average % | 89 | ||||
| Range % | 85-93 | |||||
| 1:8 | Average % | 86 | ||||
| Range % | 81-90 | |||||
回收率：
| The recovery of human PHB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 88-97 | ||||
| EDTA plasma (n=4) | 96 | 90-101 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected15002.5932.5022.5482.3877502.1702.0592.1151.9543751.3971.4191.4081.247187.50.7810.8250.8030.642940.4450.4140.4300.269470.2730.2580.2660.10523.50.2260.2120.2190.05800.1580.1630.161 | ||||||
货期：3-5 working days
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