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产品描述：人膜相关孕激素受体单元1(PGRMC1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的PGRMC1含量。PGRMC1（孕酮受体膜组分1）是一种膜相关孕酮受体，主要在肝脏和肾脏中表达。它在多种肿瘤组织中高表达，与肿瘤生长、细胞凋亡和胆固醇合成有关。PGRMC1通过调节孕酮信号通路、胆固醇合成和信号转导，在肿瘤发生发展中发挥重要作用。试剂盒检测范围为47 pg/mL-3000 pg/mL，其稳定的检测性能可支持细胞信号通路研究、疾病分子机制探索及药物靶点验证等科研场景，例如卵巢功能研究、肿瘤微环境分析或激素相关疾病模型的构建。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：PGRMC1; HPR6.6; PGRMC; Membrane-associated progesterone receptor component 1; mPR; Dap1; IZA
缩写：PGRMC1
Uniprot No.：O00264
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：47 pg/mL-3000 pg/mL
灵敏度：11.7 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human PGRMC1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 85-98 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 90-102 | |||||
| 1:4 | Average % | 93 | ||||
| Range % | 85-99 | |||||
| 1:8 | Average % | 89 | ||||
| Range % | 80-94 | |||||
回收率：
| The recovery of human PGRMC1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 100 | 96-106 | ||||
| EDTA plasma (n=4) | 95 | 90-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected30002.1242.1982.1612.04615001.4091.4151.4121.2977500.9230.9510.9370.8223750.5880.5900.5890.474187.50.3900.3840.3870.272940.2740.2860.2800.165470.1970.1900.1940.07900.1140.1160.115 | ||||||
货期：3-5 working days
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