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产品描述：人磷酸酶和肌动蛋白调节剂1(PHACTR1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的PHACTR1含量。试剂盒检测范围为23.5 pg/mL-1500 pg/mL，该产品适用于体外研究PHACTR1在心血管病理模型中的表达调控、神经退行性疾病相关机制探索，或用于细胞生物学实验中分析PHACTR1参与的细胞迁移、血管形成等信号通路活性，为分子机制研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：PHACTR1 ELISA Kit; KIAA1733 ELISA Kit; RPEL1 ELISA Kit; Phosphatase and actin regulator 1 ELISA Kit
缩写：PHACTR1
Uniprot No.：Q9C0D0
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：23.5 pg/mL-1500 pg/mL
灵敏度：5.86 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human PHACTR1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 93 | |||||
| Range % | 83-97 | ||||||
| 1:2 | Average % | 103 | |||||
| Range % | 95-107 | ||||||
| 1:4 | Average % | 90 | |||||
| Range % | 82-94 | ||||||
| 1:8 | Average % | 99 | |||||
| Range % | 90-102 | ||||||
回收率：
| The recovery of human PHACTR1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 92 | 85-96 | |||||
| EDTA plasma (n=4) | 101 | 91-105 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected15002.0511.9361.9941.8667501.2771.2441.2611.1333750.7550.7410.7480.620187.50.4270.4050.4160.288940.3150.3070.3110.183470.2520.2430.2480.12023.50.1820.1760.1790.05100.1270.1290.128 | |||||||
货期：3-5 working days
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