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产品描述：人NADH-泛醌氧化还原酶链6(MT-ND6)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的MT-ND6含量。MT-ND6是一个靶点。它在细胞呼吸链中有着重要地位，与氧化磷酸化过程相关。相关研究机制主要聚焦于其在能量代谢中的功能、基因突变对细胞生理功能的影响，以及与神经退行性疾病等病症的潜在关联，为疾病治疗提供新方向。试剂盒检测范围为23.44 pg/mL-1500 pg/mL，适用于基础科研中对线粒体功能的机制研究，例如探究疾病模型中线粒体能量代谢变化、评估基因编辑或药物干预对MT-ND6表达的影响，也可用于筛选调节线粒体呼吸链活性的化合物。其广泛的样本兼容性支持跨类型实验设计，为细胞生物学、病理生理学及代谢相关研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：MT-ND6 ELISA Kit; MTND6 ELISA Kit; NADH6 ELISA Kit; ND6 ELISA Kit; NADH-ubiquinone oxidoreductase chain 6 ELISA Kit; EC 7.1.1.2 ELISA Kit; NADH dehydrogenase subunit 6 ELISA Kit
缩写：MT-ND6
Uniprot No.：P03923
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：23.44 pg/mL-1500 pg/mL
灵敏度：5.86 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of Human MT-ND6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 84-98 | |||||
| 1:2 | Average % | 86 | ||||
| Range % | 80-92 | |||||
| 1:4 | Average % | 94 | ||||
| Range % | 85-98 | |||||
| 1:8 | Average % | 102 | ||||
| Range % | 98-106 | |||||
回收率：
| The recovery of Human MT-ND6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 88-98 | ||||
| EDTA plasma (n=4) | 89 | 83-95 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected15002.7632.8672.8152.7167502.4262.3212.3742.2753751.6751.7721.7241.625187.51.0451.1151.0800.98193.750.5270.5340.5310.43246.880.2740.2770.2760.17723.440.1880.1780.1830.08400.0980.0990.099 | ||||||
货期：3-5 working days
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