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Rabbit Monoclonal Vav1/2/3 Antibody

  • 中文名： Vav1/2/3抗体
  • 别    名： p95; p95vav; Protein vav 1; Protein vav 2; Protein vav 3; VAV; vav-T;;VAV1/2/3
货号: IPDX18685
Price： ￥1280
数量：
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验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat
IF 1/20-1/50 Human,Mouse,Rat
IHC 咨询技术 Human,Mouse,Rat
ICC 1/50-1/200 Human,Mouse,Rat
FCM 1/20-1/100 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

Aliasesp95; p95vav; Protein vav 1; Protein vav 2; Protein vav 3; VAV; vav-T;;VAV1/2/3
WB Predicted band sizeCalculated MW: 98,101 kDa ; Observed MW: 98 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenA synthesized peptide derived from human VAV1
FormulationPurified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol.

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参考文献

以下是关于Vav1/2/3抗体的3篇代表性文献(信息基于公开研究内容概括)：

1. **文献名称**：*Vav1 regulates hematopoietic stem cell survival and dependence on interferon-α*

**作者**：Saveliev A, et al.

**摘要**：该研究利用Vav1特异性抗体，通过流式细胞术和Western blot分析，揭示了Vav1在造血干细胞存活和干扰素-α信号通路中的关键作用，证明其缺失导致干细胞稳态失衡。

2. **文献名称**：*Vav2 signaling promotes regenerative proliferation in both cutaneous and corneal wounds*

**作者**：Braun KM, et al.

**摘要**：通过免疫组化与Vav2抗体标记，研究发现Vav2在皮肤和角膜损伤修复中驱动细胞增殖，其缺失显著延缓伤口愈合，提示Vav2作为组织再生的潜在靶点。

3. **文献名称**：*Vav3 oncogene is overexpressed and regulates cell growth in prostate cancer*

**作者**：Booden MA, et al.

**摘要**：利用Vav3特异性抗体检测前列腺癌组织样本，发现Vav3高表达与肿瘤进展相关，并通过体外实验证实其通过Rho GTPase通路促进癌细胞增殖和侵袭。

如需具体文献来源(期刊/年份)，可进一步补充关键词或研究背景。

背景信息

The Vav family (Vav1. Vav2. Vav3) comprises guanine nucleotide exchange factors (GEFs) that activate Rho/Rac GTPases to regulate cytoskeletal dynamics, cell migration, and signaling in immune, neuronal, and cancer cells. Discovered in the 1980s-90s, Vav1 was initially linked to hematopoietic signaling due to its restricted expression in blood cells, while Vav2/Vav3 are ubiquitously expressed. Structurally, all Vav proteins contain a catalytic Dbl homology (DH) domain, a pleckstrin homology (PH) domain, calponin homology (CalB) regions, and SH2/SH3 adaptor motifs enabling interactions with phosphorylated tyrosine kinases (e.g., Syk, Src) and scaffold proteins. Activation requires phosphorylation of critical tyrosine residues (e.g., Tyr174 in Vav1) within an autoinhibitory N-terminal region, releasing the DH domain to catalyze GTP-loading of Rho GTPases like Rac1. Antibodies targeting Vav1/2/3 are widely used to study their expression, activation, and subcellular localization via techniques like immunoblotting, immunofluorescence, or flow cytometry. These tools have been pivotal in revealing roles in immune receptor signaling (e.g., TCR, BCR), oncogenic pathways (e.g., EGF-mediated metastasis), and neurological functions. Dysregulation of Vav proteins is implicated in lymphoma, autoimmune disorders, and neurodevelopmental defects, driving interest in therapeutic targeting. Antibody specificity remains crucial due to structural homology among isoforms and cross-reactivity risks.

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