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产品描述：人膜突蛋白(MSN)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的MSN含量。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，该产品广泛用于基础研究中MSN蛋白表达动力学分析、疾病模型机制探索或药物干预效果评估，为细胞生物学、免疫学及肿瘤学等领域的体外实验提供可靠的数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Epididymis luminal protein 70 ELISA Kit; HEL70 ELISA Kit; Membrane organizing extension spike protein ELISA Kit; Membrane-organizing extension spike protein ELISA Kit; MOES_HUMAN ELISA Kit; Moesin ELISA Kit; Moesin/anaplastic lymphoma kinase fusion protein ELISA Kit; Msn ELISA Kit; MSN/ALK fusion ELISA Kit
缩写：MSN
Uniprot No.：P26038
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.81 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human MSN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 85 | |||||
| Range % | 80-90 | ||||||
| 1:2 | Average % | 104 | |||||
| Range % | 100-108 | ||||||
| 1:4 | Average % | 89 | |||||
| Range % | 85-93 | ||||||
| 1:8 | Average % | 100 | |||||
| Range % | 95-105 | ||||||
回收率：
| The recovery of human MSN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 86 | 83-89 | |||||
| EDTA plasma (n=4) | 97 | 93-100 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected20002.7442.7002.7222.62410001.9261.9021.9141.8165001.1781.2011.1901.0922500.6540.6080.6310.5331250.3880.3790.3840.28662.50.2680.2910.2800.18231.250.1830.1850.1840.08600.0960.0990.098 | |||||||
货期：3-5 working days
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