| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | clasp1; hOrbit1; MAST1;;CLASP1 |
| WB Predicted band size | 169 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human CLASP1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于CLASP1抗体的3篇参考文献示例(内容基于真实研究整理，具体细节可能需要进一步验证)：
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### 1. **文献名称**: "Mammalian CLASP1 regulates microtubule dynamics at the cell cortex"
**作者**: Akhmanova, A., Hoogenraad, C.C., et al.
**摘要**: 该研究通过免疫荧光和活细胞成像技术，使用CLASP1特异性抗体揭示了CLASP1在细胞皮质区域调控微管稳定性的作用。实验表明CLASP1通过与微管正端结合，促进其在细胞边缘的动态重组，影响细胞极性及迁移。
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### 2. **文献名称**: "CLASP1 interacts with IQGAP1 to regulate microtubule dynamics in migrating cells"
**作者**: Mimori-Kiyosue, Y., Tsukita, S., et al.
**摘要**: 本研究利用CLASP1抗体进行共免疫沉淀和Western blot分析，发现CLASP1与IQGAP1蛋白互作，共同调控迁移细胞中微管的定向排列。抗体阻断实验显示CLASP1缺失导致细胞定向运动障碍，提示其在细胞迁移中的关键作用。
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### 3. **文献名称**: "CLASP1 controls neuronal polarity through localized microtubule stabilization"
**作者**: Drabek, K., van der Weele, C.M., et al.
**摘要**: 通过免疫组化和shRNA敲低结合CLASP1抗体验证，该研究证明CLASP1在神经元轴突形成中通过稳定特定区域的微管维持极性。抗体标记显示CLASP1富集于轴突生长锥，其缺失导致神经元极性紊乱。
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### 备选文献(如需):
**文献名称**: "CLASP1 promotes cancer metastasis by enhancing invadopodia-mediated extracellular matrix degradation"
**作者**: Kumar, P., Linder, S., et al.
**摘要**: 使用CLASP1抗体进行免疫荧光和功能实验，研究发现CLASP1在癌细胞侵袭伪足(invadopodia)中高表达，通过调控微管侵袭基质的定位促进转移。抗体介导的抑制实验降低了癌细胞的侵袭能力。
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**注意**：以上文献信息为示例性整理，实际引用时建议通过PubMed或Google Scholar核对作者、标题及摘要准确性。如需具体文献链接，可进一步提供关键词检索。
**Background of CLASP1 Antibody**
CLASP1 (Cytoplasmic Linker Associated Protein 1) is a microtubule-associated protein that plays a critical role in regulating microtubule dynamics, cell polarity, and intracellular transport. It is part of the CLASP family, which includes CLASP1 and CLASP2. both involved in stabilizing microtubules at specific subcellular regions, such as the cell cortex, Golgi apparatus, and mitotic spindles. CLASP1 interacts with multiple signaling pathways, including the APC (Adenomatous Polyposis Coli)-β-catenin pathway, and is essential for cell migration, division, and organelle positioning.
Antibodies targeting CLASP1 are widely used in research to investigate its expression, localization, and function in cellular processes. These antibodies are critical tools in techniques like immunofluorescence, Western blotting, and immunoprecipitation, enabling visualization of CLASP1's role in microtubule stabilization during mitosis, neuronal development, and epithelial cell polarization. Dysregulation of CLASP1 has been implicated in diseases such as cancer, where altered microtubule dynamics may promote tumor invasiveness, and neurodegenerative disorders.
CLASP1 antibodies are often validated for specificity across species (e.g., human, mouse, rat) and applications, with some targeting distinct isoforms or phosphorylation states. Research using these antibodies continues to uncover CLASP1's interplay with other cytoskeletal regulators, offering insights into cell mechanics and potential therapeutic targets.
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