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产品描述：人β-微精原蛋白 (MSMB/PRSP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的MSMB含量。MSMB 是重要的靶点。它在前列腺等组织中表达，与肿瘤等疾病密切相关。研究机制方面，聚焦于其在信号传导通路里的作用，以及对细胞增殖、凋亡等过程的影响，探索其作为疾病诊断标志物和治疗靶点的可能性。试剂盒检测范围为2.5 ng/mL-80 ng/mL，本试剂盒可用于基础医学研究中MSMB/PRSP的表达水平检测，如前列腺疾病机制研究、肿瘤相关生物标志物探索、体外细胞模型蛋白分泌分析等场景，也可应用于组织样本中蛋白表达的定量比较实验。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Beta microseminoprotein [Precursor] ELISA Kit; Beta-microseminoprotein ELISA Kit; HPC 13 ELISA Kit; HPC13 ELISA Kit; IGBF ELISA Kit; Immunoglobulin binding factor ELISA Kit; Immunoglobulin-binding factor ELISA Kit; microseminoprotein beta ELISA Kit; Msmb ELISA Kit; MSMB_HUMAN ELISA Kit; MSP ELISA Kit; MSPB ELISA Kit; PN 44 ELISA Kit; PN44 ELISA Kit; Prostate secreted seminal plasma protein ELISA Kit; Prostate secretory protein of 94 amino acids ELISA Kit; Prostate secretory protein PSP94 ELISA Kit; PRPS ELISA Kit; PRSP ELISA Kit; PSP 57 ELISA Kit; PSP ELISA Kit; PSP-94 ELISA Kit; PSP57 ELISA Kit; PSP94 ELISA Kit; Seminal plasma beta inhibin ELISA Kit; Seminal plasma beta-inhibin ELISA Kit
缩写：MSMB
Uniprot No.：P08118
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：2.5 ng/mL-80 ng/mL
灵敏度：1.25 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<20% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human MSMB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 91 | ||||
| Range % | 85-97 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 90-102 | |||||
| 1:4 | Average % | 93 | ||||
| Range % | 86-100 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 86-101 | |||||
回收率：
| The recovery of human MSMB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 90-100 | ||||
| EDTA plasma (n=4) | 92 | 88-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2Average802.2432.2682.256401.3691.3521.361200.6720.6630.66850.2040.1940.1992.50.0920.0950.094 | ||||||
货期：3-5 working days
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