首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人B淋巴细胞抗原(CD20)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的MS4A1含量。MS4A1，也叫CD20，是B细胞特异性表面抗原，在正常及恶性B细胞上广泛表达。其研究机制主要聚焦于通过与抗体结合，引发免疫反应以杀伤B细胞。在血液系统恶性肿瘤治疗领域，针对它的靶向疗法是研究热点，可精准打击癌细胞。试剂盒检测范围为3.12 ng/mL-200 ng/mL，适用于基础科研中疾病机制探索、药物研发疗效评估及生物标志物筛选等场景，为研究B细胞功能调控、免疫治疗靶点验证及病理模型构建提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：MS4A1; CD20; B-lymphocyte antigen CD20; B-lymphocyte surface antigen B1; Bp35; Leukocyte surface antigen Leu-16; Membrane-spanning 4-domains subfamily A member 1; CD antigen CD20
缩写：MS4A1
Uniprot No.：P11836
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：3.12 ng/mL-200 ng/mL
灵敏度：0.78 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human CD20 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 96 | ||||
| Range % | 90-100 | |||||
| 1:2 | Average % | 89 | ||||
| Range % | 86-92 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 90-99 | |||||
| 1:8 | Average % | 90 | ||||
| Range % | 86-94 | |||||
回收率：
| The recovery of human CD20 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 92-103 | ||||
| EDTA plasma (n=4) | 95 | 89-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected2001.8091.8491.8291.6681001.5061.5191.5131.352501.1291.2111.1701.009250.8300.8460.8380.67712.50.5630.5790.5710.4106.250.3400.3540.3470.1863.120.2430.2640.2540.09300.1600.1620.161 | ||||||
货期：3-5 working days
×
