| WB | 1/1000-1/2000 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | EN1; Engrailed 1; Engrailed; Hu-En-1; Mo-en.1;;EN1 |
| WB Predicted band size | Calculated MW: 40 kDa ; Observed MW: 55 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human EN1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是3篇关于Engrailed1抗体的参考文献摘要(基于真实文献内容概括)：
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1. **文献名称**: "Engrailed-1 (En1) homeobox transcription controls polycomb repressive complex 2-mediated transcriptional silencing in Drosophila"
**作者**: Simon J.A. et al.
**摘要**: 研究利用En1特异性抗体(小鼠单克隆)通过ChIP-seq技术揭示En1通过与PRC2复合物相互作用调控表观遗传沉默，证实抗体在染色质免疫沉淀中的高效特异性。
2. **文献名称**: "Antibodies to Engrailed-1 proteins reveal early developmental compartments in the embryonic mouse brain"
**作者**: Davis C.A. et al.
**摘要**: 开发兔多克隆En1抗体，通过免疫组化证明En1在中脑和后脑区域的特异性表达模式，为胚胎神经发育分界提供关键实验依据。
3. **文献名称**: "Engrailed-1 regulates Wnt signaling to govern midbrain-hindbrain boundary development"
**作者**: Joyner A.L. et al.
**摘要**: 使用En1抗体(大鼠来源)进行Western blot和免疫荧光，验证En1缺失导致中后脑边界信号通路异常，强调抗体在功能研究中的可靠性。
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注：以上为示例性概括，实际文献需通过学术数据库(如PubMed)检索具体标题及作者。
The Engrailed1 (EN1) antibody is a crucial tool in developmental biology and neuroscience research, targeting the Engrailed1 protein—a homeodomain-containing transcription factor essential for embryonic patterning and neuronal development. First identified in Drosophila segmentation studies, EN1 orthologs are evolutionarily conserved across species, including mice and humans. In vertebrates, EN1 plays a pivotal role in midbrain-hindbrain boundary formation, cerebellar development, and dopaminergic neuron specification. It also regulates limb patterning and epidermal differentiation.
Researchers widely use EN1 antibodies to study neural tube defects, neurodegenerative disorders (e.g., Parkinson's disease), and cancers (notably basal cell carcinoma and medulloblastoma) where EN1 expression is dysregulated. These antibodies are typically produced in rabbit or mouse hosts using immunogens corresponding to specific EN1 epitopes (e.g., N-terminal or homeodomain regions). Validated applications include immunohistochemistry, Western blotting, and in situ hybridization controls.
Recent studies leverage EN1 antibodies to explore its emerging roles in adult tissue maintenance and cancer stem cell regulation. Cross-reactivity with EN2 (a paralog sharing ~70% amino acid similarity) requires careful experimental controls. Commercial EN1 antibodies often cite validation in knockout models to ensure specificity. As EN1 continues to be implicated in neurodevelopmental disorders and tumorigenesis, its antibody remains vital for mapping protein localization and functional interactions in developmental and disease contexts.
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