首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人激肽释放酶-5(KLK5)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆、唾液样本中的KLK5含量。KLK5 即激肽释放酶 5，是丝氨酸蛋白酶家族成员。它参与皮肤角质形成细胞的分化和脱屑等生理过程。研究发现其异常表达与皮肤疾病如特异性皮炎相关，可能通过调控炎症反应、影响皮肤屏障功能等机制发挥作用，是皮肤病研究潜在靶点。试剂盒检测范围为62.5 pg/mL-4000 pg/mL，可用于体外研究KLK5在皮肤病模型、肿瘤细胞分泌行为或唾液生物标志物筛选等场景中的动态变化，为探索相关疾病的分子机制提供定量数据支持本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：HK5 ELISA Kit; Kallikrein 5 ELISA Kit; Kallikrein L2 ELISA Kit; Kallikrein like 2 ELISA Kit; Kallikrein like protein 2 ELISA Kit; Kallikrein related peptidase 5 ELISA Kit; Kallikrein-5 ELISA Kit; Kallikrein-like protein 2 ELISA Kit; Kallikrein5 ELISA Kit; KLK 5 ELISA Kit; KLK L2 ELISA Kit; KLK-L2 ELISA Kit; KLK5 ELISA Kit; KLK5_HUMAN ELISA Kit; KLKL 2 ELISA Kit; KLKL2 ELISA Kit; SCTE ELISA Kit; Stratum corneum tryptic enzyme ELISA Kit; UNQ570/PRO1132 ELISA Kit
缩写：KLK5
Uniprot No.：Q9Y337
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates, tissue homogenates, saliva
检测范围：62.5 pg/mL-4000 pg/mL
灵敏度：15.6 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human KLK5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 94 | ||||
| Range % | 89-98 | |||||
| 1:2 | Average % | 98 | ||||
| Range % | 93-104 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 89-99 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 87-98 | |||||
回收率：
| The recovery of human KLK5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 89-98 | ||||
| EDTA plasma (n=4) | 93 | 87-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected40002.6242.5242.5742.46120002.0961.9872.0421.92910001.3811.3641.3731.2605000.7190.7420.7310.6182500.4090.4340.4220.3091250.2970.2820.2900.17762.50.2100.2150.2130.10000.1120.1130.113 | ||||||
货期：3-5 working days
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