| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CAMP GEFII; CAMPGEFII; CGEF2; EPAC2; Rapgef4;;RapGEF4 |
| WB Predicted band size | 116 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human RapGEF4 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
+ +
以下是关于Epac2抗体的3篇参考文献，包含文献名称、作者及简要摘要内容：
1. **"Role of Epac2 in insulin secretion and glucose homeostasis"**
- **作者**: Shibasaki, T., et al.
- **摘要**: 本研究利用Epac2特异性抗体，通过免疫印迹和免疫组化技术，揭示了Epac2在胰岛β细胞中的关键作用，证明其通过调控钙信号通路促进胰岛素分泌，并参与糖尿病模型的病理机制。
2. **"Epac2-dependent phosphorylation of Ras-related protein mediates neuronal excitability"**
- **作者**: Ster, J., et al.
- **摘要**: 通过Epac2抗体进行脑组织免疫荧光染色和蛋白质分析，发现Epac2在海马神经元中通过激活下游Ras相关蛋白调控突触可塑性，影响学习记忆功能。
3. **"Validation of a high-affinity anti-Epac2 antibody for cAMP signaling studies"**
- **作者**: Dao, K.K., et al.
- **摘要**: 该文献详细描述了一种高特异性Epac2抗体的开发与验证，证明其在流式细胞术、免疫沉淀等实验中的可靠性，并用于检测不同组织中Epac2的表达差异及cAMP信号动态。
以上文献均涉及Epac2抗体的实验应用，涵盖其在代谢、神经科学及抗体开发中的研究。
The Epac2 (Exchange Protein Activated by cAMP 2) antibody is a critical tool for studying the cAMP signaling pathway, which regulates diverse cellular processes such as metabolism, secretion, and gene expression. Epac2. also known as RAPGEF4. is a guanine nucleotide exchange factor activated by cyclic AMP (cAMP), functioning independently of the classical protein kinase A (PKA) pathway. It modulates intracellular signaling by activating small GTPases like Rap1 and Rap2. thereby influencing cell adhesion, differentiation, and synaptic plasticity.
Epac2 is highly expressed in brain regions (e.g., hippocampus, hypothalamus), pancreatic β-cells, and endocrine tissues, making it a focus in diabetes and neurological disorder research. In pancreatic β-cells, Epac2 mediates cAMP-enhanced insulin secretion, linking it to type 2 diabetes pathophysiology. Dysregulation of Epac2 has also been implicated in neuropsychiatric conditions, including autism spectrum disorders and Alzheimer’s disease.
Antibodies targeting Epac2 enable researchers to detect its expression, localization, and interaction partners via techniques like Western blotting, immunohistochemistry, and co-immunoprecipitation. Specificity and validation (e.g., knockout controls) are crucial due to structural similarities with Epac1 (RAPGEF3). These antibodies have advanced studies on cAMP-Epac2 signaling in disease models, drug discovery, and mechanistic explorations of cellular responses to hormones and neurotransmitters. Their development underscores the importance of Epac2 as a therapeutic target and biomarker in metabolic and neurological diseases.
×
