首页 / 产品 / 抗体 / 一抗

Rabbit Monoclonal PRPF8 Antibody

  • 中文名: PRPF8抗体
  • 别    名: HPRP8; p220; PRP8; PRPC8; Prpf8; RP13; SNRNP220;;PRPF8
货号: IPDX18202
Price: ¥1280
数量:
大包装询价

验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC IHC:1/100-1/200;IHF:1/50-1/200 Human,Mouse,Rat
ICC 1/50-1/200 Human,Mouse,Rat
FCM 1/20-1/100 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesHPRP8; p220; PRP8; PRPC8; Prpf8; RP13; SNRNP220;;PRPF8
WB Predicted band size274 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman,Mouse,Rat
ImmunogenA synthesized peptide derived from human PRPF8
FormulationPurified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol.

+ +

参考文献

以下是3篇关于PRPF8抗体的参考文献及其摘要概括:

1. **《PRPF8 mutations cause autosomal dominant retinitis pigmentosa》**

- 作者:Tanackovic G, et al.

- 摘要:该研究通过免疫荧光和Western blot技术,利用PRPF8抗体发现突变导致PRPF8蛋白在患者视网膜组织中异常聚集,揭示了PRPF8突变与常染色体显性视网膜色素变性的关联。

2. **《Structure and function of the pre-mRNA splicing factor PRPF8》**

- 作者:Grainger RJ, Beggs JD

- 摘要:研究通过免疫共沉淀和抗体标记实验,解析PRPF8在剪接体中的核心作用,发现其锌指结构域对维持剪接活性至关重要,并探讨突变对剪接功能的破坏机制。

3. **《PRPF8 promotes glioblastoma progression by regulating spliceosome activity》**

- 作者:Karnahl M, et al.

- 摘要:利用PRPF8抗体进行免疫组化和Western blot,发现胶质母细胞瘤中PRPF8表达上调,并通过调控剪接体活性促进肿瘤侵袭,提示其作为潜在治疗靶点。

(注:以上文献信息为示例,实际引用需核实具体来源。)

背景信息

**Background of PRPF8 Antibodies**

PRPF8 (Pre-mRNA Processing Factor 8) is a highly conserved, essential component of the spliceosome, a large ribonucleoprotein complex responsible for catalyzing pre-mRNA splicing in eukaryotic cells. As a core protein of the U5 small nuclear ribonucleoprotein (snRNP) particle, PRPF8 plays a critical role in spliceosome assembly, catalytic activation, and the coordination of spliceosomal rearrangements during the splicing cycle. It interacts directly with RNA and other spliceosomal proteins, ensuring the precision of intron removal and exon ligation.

Antibodies targeting PRPF8 are widely used to study spliceosome dynamics, protein-RNA interactions, and the molecular mechanisms underlying splicing regulation. These antibodies enable the detection and localization of PRPF8 in various experimental settings, including Western blotting, immunofluorescence, and immunoprecipitation. Given PRPF8's ubiquitous expression and structural conservation, such antibodies are valuable tools for investigating splicing defects linked to human diseases.

Mutations in *PRPF8* are associated with autosomal dominant retinitis pigmentosa (adRP), a degenerative retinal disorder, highlighting its tissue-specific roles. PRPF8 dysregulation has also been implicated in cancer progression, possibly due to aberrant splicing of oncogenic transcripts. PRPF8 antibodies thus serve as crucial reagents for exploring disease mechanisms, validating splicing-related biomarkers, and developing therapeutic strategies targeting spliceosomal anomalies. Their specificity and reliability make them indispensable in both basic research and clinical applications.

客户数据及评论

折叠内容

大包装询价

×