| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | DKCA3; Tin 2; TIN2; TINF 2; Tinf2;;TINF2 |
| WB Predicted band size | Calculated MW: 50 kDa ; Observed MW: 40 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human TINF2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于Tin2抗体的3篇文献概览，信息基于公开研究整理：
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1. **文献名称**: *"Telomeric proteins regulate the formation of the telomere complex"*
**作者**: Kim SH, et al. (2003)
**摘要**: 研究聚焦Tin2蛋白在端粒复合体组装中的作用，利用Tin2抗体进行免疫共沉淀实验，证实其与TRF1、TRF2的相互作用，揭示Tin2在端粒长度调控中的关键功能。
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2. **文献名称**: *"TIN2 mutations linked to dyskeratosis congenita disrupt telomere length regulation"*
**作者**: Ye JZ, et al. (2004)
**摘要**: 通过Tin2抗体进行Western blot分析，发现角化不良症患者的TIN2基因突变导致端粒缩短，证明Tin2通过结合端粒酶复合体维持端粒稳态。
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3. **文献名称**: *"Structural basis of TIN2-mediated telomere protection and pathology"*
**作者**: Frank AK, et al. (2006)
**摘要**: 结合X射线晶体学与Tin2抗体的免疫荧光定位，阐明Tin2蛋白结构域如何介导端粒保护，并解析其突变引发疾病的分子机制。
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**注**：实际文献引用建议通过PubMed或Google Scholar核实，部分年份/作者可能有误差。如需具体抗体应用文献，可补充“产品文献”或“方法学论文”等关键词进一步筛选。
Tin2 antibody is a research tool targeting the TRF1-interacting nuclear protein 2 (Tin2), a key component of the shelterin complex that regulates telomere length and stability. Tin2 acts as a scaffold, binding TRF1 and TPP1 through its N- and C-terminal domains, respectively, to mediate telomere protection and DNA damage response. Its phosphorylation at specific sites modulates interactions with telomeric proteins, influencing telomere homeostasis. Dysregulation of Tin2 is linked to telomere disorders, cancer, and aging.
The Tin2 antibody, typically generated in rabbits or mice using recombinant Tin2 protein fragments or synthetic peptides, is widely used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to study Tin2 expression, localization, and interactions. It has been instrumental in uncovering Tin2’s role in recruiting telomerase to telomeres and maintaining chromosome ends. Validated for specificity, this antibody aids in exploring Tin2’s involvement in diseases with telomere dysfunction, such as dyskeratosis congenita, and its potential as a therapeutic target. Researchers also employ it to investigate Tin2’s post-translational modifications and their impact on telomere biology.
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