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产品描述：人KELCH样ECH关联蛋白1(KEAP1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的KEAP1含量。KEAP1是一种与氧化应激和亲电性应激损伤相关的防御蛋白，其与Nrf2组成细胞防御体系。在正常情况下，Keap1结合Nrf2并促进其降解，维持细胞内Nrf2低水平。当细胞受到应激时，Keap1阻止Nrf2降解，激活Nrf2进入细胞核，启动抗氧化反应，保护细胞免受损伤。KEAP1在肿瘤发生发展中起重要作用，其表达水平与肿瘤耐药性相关，是肿瘤治疗的潜在靶点。试剂盒检测范围为25 pg/mL-1600 pg/mL，广泛应用于基础科研领域，如氧化应激相关信号通路研究、疾病模型构建中KEAP1/Nrf2通路的动态监测，或药物开发过程中评估化合物对KEAP1表达的影响。其高灵敏度和宽检测范围尤其适合微量样本或低丰度靶标分析，例如细胞信号转导研究或组织特异性蛋白表达检测。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Cytosolic inhibitor of Nrf2 ELISA Kit; INrf 2 ELISA Kit; INrf2 ELISA Kit; Keap 1 ELISA Kit; KEAP1 ELISA Kit; KEAP1_HUMAN ELISA Kit; Kelch like ECH associated protein 1 ELISA Kit; Kelch like family member 19 ELISA Kit; Kelch like protein 19 ELISA Kit; Kelch-like ECH-associated protein 1 ELISA Kit; Kelch-like protein 19 ELISA Kit; KIAA0132 ELISA Kit; KLHL 19 ELISA Kit; KLHL19 ELISA Kit; MGC10630 ELISA Kit; MGC1114 ELISA Kit; MGC20887 ELISA Kit; MGC4407 ELISA Kit; MGC9454 ELISA Kit
缩写：KEAP1
Uniprot No.：Q14145
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：25 pg/mL-1600 pg/mL
灵敏度：6.25 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Epigenetics and Nuclear Signaling
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human KEAP1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 92 | |||||
| Range % | 89-96 | ||||||
| 1:2 | Average % | 97 | |||||
| Range % | 93-103 | ||||||
| 1:4 | Average % | 95 | |||||
| Range % | 89-99 | ||||||
| 1:8 | Average % | 92 | |||||
| Range % | 87-98 | ||||||
回收率：
| The recovery of human KEAP1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 103 | 95-107 | |||||
| EDTA plasma (n=4) | 88 | 83-94 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected16002.9682.8562.9122.7778002.1212.2222.1722.0374001.4231.4851.4541.3192000.8640.8470.8560.7211000.5530.5370.5450.410500.3720.3840.3780.243250.2420.2470.2450.11000.1360.1340.135 | |||||||
货期：3-5 working days
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