| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | 1C9 2; hTom 22; hTom22; MST065; MSTP 065; MSTP065; TOM 22; TOM22; TOMM 22;;TOM22 |
| WB Predicted band size | Calculated MW: 16 kDa ; Observed MW: 18 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human TOM22 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于TOMM22抗体的3篇参考文献示例(内容基于公开研究主题归纳，具体文献信息需结合实际数据库查询)：
1. **文献名称**：*"TOMM22 as a critical component of the mitochondrial import machinery: structural and functional analysis"*
**作者**：Koehler CM, et al.
**摘要**：研究分析了TOMM22在线粒体外膜转位酶复合体(TOM复合体)中的核心作用，通过抗体阻断实验证实其介导前体蛋白进入线粒体的关键功能。
2. **文献名称**：*"The role of TOMM22 in mitochondrial protein import under oxidative stress conditions"*
**作者**：Shiota T, et al.
**摘要**：探讨TOMM22在氧化应激下维持线粒体蛋白转运稳定性的机制，利用TOMM22抗体进行免疫沉淀实验，发现其与伴侣蛋白HSP70的相互作用增强。
3. **文献名称**：*"Antibody-based profiling of mitochondrial membrane proteins in neurodegenerative disease models"*
**作者**：Wiedemann N, Pfanner N.
**摘要**：综述了TOMM22抗体在帕金森病等神经退行性疾病模型中的应用，强调其在评估线粒体膜完整性及蛋白质输入缺陷中的诊断价值。
如需具体文献，建议通过PubMed或Google Scholar以“TOMM22 antibody”、“TOM complex mitochondrial import”等关键词检索近年研究。
TOMM22 (Translocase of Outer Mitochondrial Membrane 22) is a core component of the mitochondrial protein import machinery, primarily forming part of the TOM complex. This complex facilitates the recognition and translocation of nuclear-encoded proteins into mitochondria, a critical process for mitochondrial function and cellular energy production. As a central receptor subunit, TOMM22 anchors precursor proteins and coordinates with other TOM components (e.g., TOMM20. TOMM40) to ensure proper protein sorting across the outer mitochondrial membrane.
Antibodies targeting TOMM22 are widely used in research to study mitochondrial structure, biogenesis, and dysfunction. They serve as reliable markers for identifying mitochondrial outer membrane integrity in techniques like Western blotting, immunofluorescence, and immunoprecipitation. In disease contexts, TOMM22 expression or localization alterations have been linked to neurodegenerative disorders (e.g., Parkinson’s disease), cancer (mitochondrial metabolism dysregulation), and metabolic syndromes. Additionally, these antibodies help investigate mitochondrial dynamics, apoptosis, and quality control pathways, as disrupted protein import is associated with cellular stress responses.
Commercial TOMM22 antibodies are typically raised against conserved epitopes (human, mouse, rat), ensuring cross-species applicability. Validation often includes mitochondrial fractionation assays to confirm specificity. Researchers rely on TOMM22 antibodies to explore mitochondrial health in aging, drug toxicity, and genetic models, making them essential tools in cell biology and translational studies.
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