| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CLSD; sec23a;;Sec23A |
| WB Predicted band size | 86 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human Sec23A |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于SEC23抗体的示例参考文献(内容为模拟，仅供参考)：
1. **文献名称**：COPII-coated vesicle formation reconstituted with purified coat proteins and chemically defined liposomes
**作者**：Barlowe, C., et al.
**摘要**：该研究利用纯化的COPII组分(包括SEC23/24复合体)在体外重构囊泡形成，通过SEC23抗体验证蛋白在脂质体上的定位，证实其在内质网出泡过程中的关键作用。
2. **文献名称**：Mutations in SEC23B cause congenital dyserythropoietic anemia type II
**作者**：Schwarz, K., et al.
**摘要**：通过免疫印迹和免疫荧光技术(使用SEC23特异性抗体)，发现SEC23B基因突变导致红细胞发育异常，揭示其与遗传性贫血的关联。
3. **文献名称**：Structural basis for cargo regulation of COPII coat assembly
**作者**：Stagg, S.M., et al.
**摘要**：结合冷冻电镜和SEC23抗体标记，解析了COPII复合体结合不同运输货物时的构象变化，阐明SEC23在囊泡包装中的调控机制。
4. **文献名称**：Aberrant SEC23A expression promotes tumor progression via EMT in pancreatic cancer
**作者**：Li, X., et al.
**摘要**：采用SEC23抗体进行组织芯片分析，发现SEC23A在胰腺癌中高表达，并通过上皮-间质转化(EMT)促进肿瘤侵袭转移。
建议通过PubMed或Google Scholar以“SEC23 antibody”、“COPII SEC23”等关键词检索最新文献以获取真实数据。
SEC23 antibody targets the SEC23 protein, a critical component of the COPII (coat protein complex II) vesicle coat machinery involved in endoplasmic reticulum (ER)-to-Golgi transport. SEC23. along with SEC24. forms the inner layer of COPII-coated vesicles, facilitating cargo selection and vesicle budding from the ER. It acts as a GTPase-activating protein (GAP) for SAR1. promoting GTP hydrolysis and vesicle uncoating. Mammalian cells express two paralogs, SEC23A and SEC23B, which share structural similarity but exhibit tissue-specific expression and functional roles. SEC23B mutations are linked to congenital dyserythropoietic anemia type II (CDA II), underscoring its importance in erythropoiesis.
SEC23 antibodies are widely used in cell biology research to study secretory pathway dynamics, ER stress, and COPII-mediated trafficking. They are employed in techniques like Western blotting, immunofluorescence, and immunoprecipitation to localize SEC23. assess expression levels, or investigate interactions with COPII components. Commercially available antibodies are often validated for specificity across human, mouse, and rat samples. Researchers also utilize SEC23 antibodies to explore pathological mechanisms in cancers (e.g., SEC23A suppression in tumor metastasis) or genetic disorders tied to COPII dysfunction. Proper controls, such as knockout cell lines, are essential to confirm antibody specificity due to paralog homology.
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