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产品描述：人间α-胰蛋白酶抑制剂重链H2(ITIH2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的ITIH2含量。试剂盒检测范围为3.12 μg/mL-200 μg/mL，适用于基础医学研究中ITIH2蛋白水平的定量分析，如炎症性疾病机制探究、肿瘤微环境研究、代谢相关疾病动物模型评估等科研场景，也可用于比较不同组织样本中ITIH2的表达差异，为相关分子调控网络研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：AI747202 ELISA Kit; H2P ELISA Kit; IGHEP2 ELISA Kit; Inter alpha (globulin) inhibitor H2 ELISA Kit; Inter alpha globulin inhibitor H2 polypeptide ELISA Kit; Inter alpha trypsin inhibitor complex component II ELISA Kit; Inter alpha trypsin inhibitor heavy chain H2 ELISA Kit; Inter-alpha-inhibitor heavy chain 2 ELISA Kit; Inter-alpha-trypsin inhibitor complex component II ELISA Kit; Inter-alpha-trypsin inhibitor heavy chain H2 ELISA Kit; Intin2 ELISA Kit; ITI HC2 ELISA Kit; ITI heavy chain H2 ELISA Kit; ITI-HC2 ELISA Kit; Itih 2 ELISA Kit; Itih2 ELISA Kit; ITIH2_HUMAN ELISA Kit; OTTHUMP00000019058 ELISA Kit; OTTHUMP00000019059 ELISA Kit; OTTMUSP00000012019 ELISA Kit; RP23 119N4.3 ELISA Kit; Serum derived hyaluronan associated protein ELISA Kit; Serum-derived hyaluronan-associated protein ELISA Kit; SHAP ELISA Kit
缩写：ITIH2
Uniprot No.：P19823
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：3.12 μg/mL-200 μg/mL
灵敏度：0.78 μg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human ITIH2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:5 | Average % | 91 | ||||
| Range % | 86-94 | |||||
| 1:10 | Average % | 97 | ||||
| Range % | 90-104 | |||||
| 1:20 | Average % | 101 | ||||
| Range % | 96-107 | |||||
| 1:40 | Average % | 89 | ||||
| Range % | 84-94 | |||||
回收率：
| The recovery of human ITIH2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 88-95 | ||||
| EDTA plasma (n=4) | 102 | 97-107 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| μg/mlOD1OD2AverageCorrected2002.4392.5552.4972.3121001.8671.7281.7981.613501.2211.2651.2431.058250.7510.7570.7540.56912.50.4120.4220.4170.2326.250.3180.3360.3270.1423.120.2720.2810.2770.09200.1830.1860.185 | ||||||
货期：3-5 working days
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