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产品描述：人间α胰蛋白酶抑制剂重链H3(ITIH3)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的ITIH3含量。ITIH3是一种在血清中作为透明质酸载体的蛋白质，同时促进与各种基质蛋白的相互作用。这些相互作用调节透明质酸的定位、合成和降解，对多种细胞活动至关重要。ITIH3在生理和病理过程中发挥重要作用，如炎症、肿瘤发生、神经发育和精神疾病等。试剂盒检测范围为78.13 ng/mL-5000 ng/mL，该产品适用于基础医学研究中ITIH3蛋白表达水平分析，例如肝脏功能研究、炎症相关疾病模型构建、生物标志物筛选等领域，可为探索ITIH3在细胞黏附、组织修复中的分子机制提供可靠数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：ITIH3 ELISA Kit; Inter-alpha-trypsin inhibitor heavy chain H3 ELISA Kit; ITI heavy chain H3 ELISA Kit; ITI-HC3 ELISA Kit; Inter-alpha-inhibitor heavy chain 3 ELISA Kit; Serum-derived hyaluronan-associated protein ELISA Kit; SHAP ELISA Kit
缩写：ITIH3
Uniprot No.：Q06033
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：78.13 ng/mL-5000 ng/mL
灵敏度：19.53 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human TIH3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:20 | Average % | 92 | ||||
| Range % | 86-99 | |||||
| 1:40 | Average % | 97 | ||||
| Range % | 92-103 | |||||
| 1:80 | Average % | 86 | ||||
| Range % | 80-89 | |||||
| 1:160 | Average % | 90 | ||||
| Range % | 83-99 | |||||
回收率：
| The recovery of human TIH3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 87-95 | ||||
| EDTA plasma (n=4) | 95 | 90-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2Average50000.0830.0850.08425000.1390.1470.14312500.2230.2200.2226250.3290.3470.338312.50.5720.6140.593156.30.8140.8490.83278.131.2281.2591.24402.0812.2262.154 | ||||||
货期：3-5 working days
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