首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人β2整合素(ITG β2/CD11+CD18)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的ITGB2含量。ITGB2 即整合素 β2，是一种跨膜糖蛋白，在白细胞表面表达。它介导白细胞与内皮细胞、病原体等的黏附作用，参与炎症反应、免疫应答等生理病理过程。目前研究聚焦于其信号传导机制及作为靶点开发抗炎、免疫调节药物。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，在免疫性疾病和感染机制研究中具有重要价值；适用于免疫学基础研究、炎症相关疾病模型分析、药物干预对整合素通路影响等科研领域；尤其适合检测血液及组织裂解液等复杂样本中的低丰度靶标，为探索β2整合素在免疫调控中的作用提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：ITGB2; CD18; MFI7; Integrin beta-2; Cell surface adhesion glycoproteins LFA-1/CR3/p150,95 subunit beta; Complement receptor C3 subunit beta; CD antigen CD18
缩写：ITGB2
Uniprot No.：P05107
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human ITG β2/CD11+CD18CD18 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 90 | ||||
| Range % | 85-97 | |||||
| 1:2 | Average % | 91 | ||||
| Range % | 84-99 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 90-98 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 86-102 | |||||
回收率：
| The recovery of human ITG β2/CD11+CD18CD18 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-98 | ||||
| EDTA plasma (n=4) | 96 | 87-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 10001.6311.6091.6201.523 5001.3651.3181.3421.245 2500.9520.9430.9480.851 1250.6500.6350.6430.546 62.50.3810.3770.3790.282 31.20.2670.2590.2630.166 15.60.1850.1910.1880.091 00.0950.0990.097 | ||||||
货期：3-5 working days
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