首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人白介素1受体相关激酶-4(IRAK-4)酶联免疫试剂盒,为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IRAK4含量。IRAK4在免疫信号传导中发挥重要作用。它参与Toll样受体和白细胞介素-1受体介导的信号通路，激活下游转录因子。相关研究围绕抑制其活性展开，期望开发治疗自身免疫病、炎症性疾病等的药物，有望为相关疾病提供新治疗策略。试剂盒检测范围为6.25 pg/mL-400 pg/mL，适用于科研场景中炎症信号通路研究、药物靶点筛选或免疫调节机制探索，通过精准定量IRAK-4蛋白表达水平，为先天免疫系统功能评估及疾病模型构建提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：IL-1 receptor-associated kinase 4 ELISA Kit; Interleukin 1 receptor associated kinase 4 mutant form 1 ELISA Kit; Interleukin-1 receptor-associated kinase 4 ELISA Kit; Interleukin1 receptor associated kinase 4 ELISA Kit; IPD1 ELISA Kit; IRAK 4 ELISA Kit; IRAK-4 ELISA Kit; IRAK4 ELISA Kit; IRAK4 mutated form 1 ELISA Kit; IRAK4_HUMAN ELISA Kit; LOC 51135 ELISA Kit; NY REN 64 ELISA Kit; NY REN 64 antigen ELISA Kit; NY-REN-64 ELISA Kit; REN64 ELISA Kit; Renal carcinoma antigen NY-REN-64 ELISA Kit
缩写：IRAK4
Uniprot No.：Q9NWZ3
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：6.25 pg/mL-400 pg/mL
灵敏度：1.56 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human IRAK-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:5 | Average % | 92 | |||||
| Range % | 86-96 | ||||||
| 1:10 | Average % | 107 | |||||
| Range % | 103-111 | ||||||
| 1:20 | Average % | 92 | |||||
| Range % | 87-98 | ||||||
| 1:40 | Average % | 88 | |||||
| Range % | 84-93 | ||||||
回收率：
| The recovery of human IRAK-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 87 | 83-92 | |||||
| EDTA plasma (n=4) | 102 | 97-105 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected4002.9872.9202.9542.8672002.4852.3152.4002.3131001.5781.5641.5711.484500.8520.8430.8480.761250.4730.4670.4700.38312.50.2550.2450.2500.1636.250.1690.1610.1650.07800.0870.0860.087 | |||||||
货期：3-5 working days
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