| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | DPDE4; PDE 4B; PDE IVB; PDE32; Pde4b; PDE4B5; PDEIVB; Phosphodiesterase 4B;;PDE4B |
| WB Predicted band size | Calculated MW: 83 kDa ; Observed MW: 95 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | A synthesized peptide derived from human PDE4B |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于PDE4B抗体的3篇示例文献(内容为示例性概括，非真实文献)：
1. **"Phosphodiesterase 4B in macrophages: A potential target for anti-inflammatory therapy"**
*作者：Smith A, et al.*
**摘要**：本研究通过Western blot和免疫组化技术，利用PDE4B特异性抗体证实其在巨噬细胞中的高表达。实验表明，抑制PDE4B活性可降低LPS诱导的炎症因子释放，提示其作为炎症性疾病治疗靶点的潜力。
2. **"PDE4B antibody-based screening identifies novel inhibitors for neurodegenerative disease"**
*作者：Chen L, et al.*
**摘要**：开发了一种基于PDE4B抗体的高通量筛选方法，用于鉴定选择性PDE4B抑制剂。研究通过免疫沉淀验证抑制剂与PDE4B的结合，并在阿尔茨海默病模型中观察到认知功能改善。
3. **"Localization and functional analysis of PDE4B isoforms in neuronal cells"**
*作者：Johnson R, et al.*
**摘要**：利用PDE4B特异性抗体进行免疫荧光染色，揭示了PDE4B不同亚型在神经元中的亚细胞定位差异，并发现特定亚型与cAMP信号调控及突触可塑性密切相关。
*注：以上为模拟示例，实际文献需通过PubMed、Google Scholar等平台检索。*
PDE4B (phosphodiesterase 4B) is a member of the PDE4 enzyme family that specifically hydrolyzes cyclic adenosine monophosphate (cAMP), a critical secondary messenger regulating diverse cellular processes, including inflammation, immune responses, and neuronal signaling. PDE4B is highly expressed in immune cells, the brain, and other tissues, and its activity modulates cAMP levels, thereby influencing pathways linked to cell proliferation, differentiation, and apoptosis. Dysregulation of PDE4B has been implicated in various diseases, such as chronic obstructive pulmonary disease (COPD), asthma, depression, and certain cancers, making it a target for therapeutic intervention.
PDE4B antibodies are essential tools for studying the enzyme's expression, localization, and function in both physiological and pathological contexts. They enable detection of PDE4B protein levels in tissues or cell lysates via techniques like Western blotting, immunohistochemistry, or flow cytometry. Additionally, these antibodies aid in elucidating PDE4B's role in signaling cascades and its interaction with other molecules. Research using PDE4B-specific antibodies has highlighted its involvement in inflammatory responses, where inhibition of PDE4B enhances cAMP signaling, potentially suppressing pro-inflammatory cytokine production.
The development of selective PDE4B inhibitors, such as roflumilast, underscores its clinical relevance, though challenges remain in minimizing side effects associated with off-target PDE4 family inhibition. PDE4B antibodies continue to support drug discovery and mechanistic studies, bridging gaps in understanding cAMP-mediated pathways and their therapeutic exploitation.
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