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Rabbit Monoclonal NDUFS1 Antibody

  • 中文名： NDUFS1抗体
  • 别    名： CI-75kD; NDUFS1; PRO1304;;NDUFS1
货号: IPDX18050
Price： ￥1280
数量：
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验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat
IF 1/20-1/50 Human,Mouse,Rat
IHC IHC:1/100-1/200;IHF:1/50-1/200 Human,Mouse,Rat
ICC 1/50-1/200 Human,Mouse,Rat
FCM 1/20-1/100 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesCI-75kD; NDUFS1; PRO1304;;NDUFS1
WB Predicted band sizeCalculated MW: 79 kDa ; Observed MW: 75 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman,Mouse,Rat
ImmunogenA synthesized peptide derived from human NDUFS1
FormulationPurified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol.

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参考文献

以下是3篇关于NDUFS1抗体的虚构参考文献示例，格式为文献名称、作者及摘要概括：

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1. **"NDUFS1 deficiency in mitochondrial complex I assembly: Insights from antibody-based proteomic analysis"**

*作者：Smith J, et al. (Journal of Mitochondrial Research, 2018)*

**摘要**：本研究利用NDUFS1特异性抗体，通过Western blot和免疫荧光技术，揭示了NDUFS1亚基在线粒体复合体I组装中的关键作用。实验表明，NDUFS1缺失导致复合体I稳定性下降，并与 Leigh 综合征患者的细胞模型中线粒体呼吸链功能障碍相关。

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2. **"Antibody validation for NDUFS1 in neurodegenerative disease models"**

*作者：Chen L, et al. (Neurochemistry International, 2020)*

**摘要**：通过比较多种商业NDUFS1抗体的特异性，本研究验证了其在帕金森病小鼠脑组织中的适用性。免疫组化结果显示，NDUFS1蛋白在黑质区神经元中表达显著降低，提示其与多巴胺能神经元退行性变存在关联。

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3. **"Targeting NDUFS1 in cancer metabolism: Insights from CRISPR and antibody-mediated knockdown"**

*作者：Gomez-Ramos A, et al. (Oncogene, 2021)*

**摘要**：结合CRISPR基因编辑和NDUFS1抗体介导的蛋白检测，研究发现NDUFS1表达抑制会削弱肿瘤细胞的氧化磷酸化能力，并增强其对糖酵解的依赖，为靶向线粒体代谢的癌症治疗提供了新思路。

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注：以上文献为模拟示例，实际引用需以真实出版物为准。如需真实文献，建议通过PubMed或Google Scholar以关键词“NDUFS1 antibody”+“应用场景(如Western blot/immunohistochemistry)”检索。

背景信息

The NDUFS1 antibody is a crucial tool for studying the NADH:ubiquinone oxidoreductase core subunit S1 (NDUFS1), a 75 kDa protein encoded by nuclear DNA and localized to the mitochondrial inner membrane. As a core component of mitochondrial Complex I (NADH dehydrogenase), NDUFS1 plays a vital role in the electron transport chain by facilitating NADH oxidation and proton gradient establishment for ATP synthesis. This iron-sulfur cluster-containing subunit is essential for Complex I assembly and catalytic activity. Antibodies targeting NDUFS1 are widely used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to assess protein expression, localization, and integrity in research contexts. They are particularly valuable in investigating mitochondrial disorders, such as Leigh syndrome and cardiomyopathy, where NDUFS1 mutations disrupt oxidative phosphorylation. Additionally, these antibodies aid in studying cancer metabolism, neurodegenerative diseases (e.g., Parkinson's), and aging processes linked to mitochondrial dysfunction. Commercial NDUFS1 antibodies are typically validated for species cross-reactivity (human, mouse, rat) and require proper mitochondrial fractionation for accurate detection. Recent studies also utilize them to explore metabolic adaptations in hypoxia and evaluate therapeutic responses targeting cellular energetics.

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