| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | FASPS; FASPS1; hPER 2; hPER2; KIAA0347; PER2; Period 2;;PER2 |
| WB Predicted band size | 137 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human PER2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是3篇与PER2抗体相关的文献摘要示例(文献信息为虚拟概括，仅作参考)：
1. **文献名称**：*"Circadian Regulator PER2 Potentiates Oncogene-Induced Apoptosis in Colorectal Cancer Cells"*
**作者**：Smith A, et al.
**摘要**：研究通过免疫组化(使用兔源PER2多克隆抗体)分析了结直肠癌组织中PER2蛋白的昼夜表达模式，发现其表达下降与肿瘤进展相关，并验证了抗体在Western blot中的特异性。
2. **文献名称**：*"Antibody Validation and Localization of PER2 in the Mammalian Hypothalamus"*
**作者**：Jones B, et al.
**摘要**：系统性比较了多种市售PER2抗体的特异性(包括小鼠单克隆抗体SC-25363)，通过siRNA敲低实验验证抗体可靠性，并利用免疫荧光证实PER2在下丘脑视交叉上核的节律性表达。
3. **文献名称**：*"PER2 Methylation Status and Protein Expression in Breast Cancer Prognosis"*
**作者**：Chen L, et al.
**摘要**：采用免疫组织化学(兔抗人PER2抗体，货号AB1234)检测乳腺癌患者样本，发现PER2蛋白低表达与不良预后显著相关，并通过质谱分析确认抗体表位特异性。
注：实际文献需通过PubMed/Google Scholar检索关键词“PER2 antibody validation”、“PER2 immunohistochemistry”等获取。建议优先选择近5年、高引用的研究，并注意抗体货号与实验应用的匹配性。
The PER2 antibody is a crucial tool in circadian rhythm research, targeting the Period Circadian Regulator 2 (PER2) protein, a core component of the mammalian biological clock. PER2. encoded by the *PER2* gene, operates within the transcription-translation feedback loop (TTFL) that drives 24-hour circadian oscillations. It interacts with other clock proteins like CLOCK and BMAL1 to regulate rhythmic gene expression, influencing physiological processes such as sleep-wake cycles, metabolism, and hormone secretion.
PER2 antibodies are typically developed in hosts like rabbits or mice using immunogenic peptides or recombinant PER2 protein fragments. These antibodies enable researchers to detect PER2 expression levels, localization, and post-translational modifications via techniques including Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). They are widely used to study circadian disruptions in diseases (e.g., sleep disorders, cancer, metabolic syndromes) and to explore PER2's roles in cellular responses like DNA repair and apoptosis.
Validation of PER2 antibodies is critical due to potential cross-reactivity with homologous proteins (e.g., PER1 or PER3) or isoform-specific epitopes. Knockout controls and specificity tests are recommended. Commercial PER2 antibodies vary in performance across applications, emphasizing the need for protocol optimization. Recent studies also utilize PER2 antibodies in chronotherapeutic research, aiming to align treatments with circadian phases for enhanced efficacy. Overall, these antibodies remain indispensable for unraveling the molecular basis of circadian biology and its health implications. (298 words)
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