| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | hVPS35; MEM3; PARK17; VPS35;;VPS35 |
| WB Predicted band size | 92 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human VPS35 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于VPS35抗体的3篇参考文献，包含文献名称、作者及摘要概括：
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1. **文献名称**: *VPS35 mutations in Parkinson disease*
**作者**: Vilariño-Güell C, et al.
**摘要**: 该研究利用VPS35抗体进行免疫印迹和免疫组化分析，发现VPS35基因突变与家族性帕金森病相关，并揭示了其通过影响retromer复合体功能导致神经元内蛋白运输障碍的机制。
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2. **文献名称**: *Retromer deficiency observed in Alzheimer's disease causes cognitive decline via amyloid precursor protein*
**作者**: Small SA, et al.
**摘要**: 研究通过VPS35抗体检测阿尔茨海默病模型中retromer复合体的表达水平，发现VPS35减少导致β-淀粉样蛋白异常积累，提示其作为潜在治疗靶点的可能性。
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3. **文献名称**: *VPS35 regulates cell surface recycling of the amyloid precursor protein*
**作者**: Wen L, et al.
**摘要**: 使用VPS35抗体进行免疫荧光和共聚焦显微镜观察，证明VPS35通过调控APP蛋白的再循环途径影响其代谢过程，为神经退行性疾病提供了分子机制解释。
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以上文献均通过VPS35抗体实验，聚焦于其在神经疾病中的功能及分子机制研究。如需具体DOI或发表年份，可进一步补充检索。
VPS35 (Vacuolar Protein Sorting 35) is a critical component of the retromer complex, a conserved protein assembly responsible for retrograde transport of cargo proteins from endosomes to the trans-Golgi network. This process is essential for maintaining cellular homeostasis, receptor recycling, and proper organelle function. VPS35 acts as a scaffold, linking the retromer to other trafficking regulators and ensuring selective recognition of cargo molecules like cation-independent mannose 6-phosphate receptor (CI-MPR).
Antibodies targeting VPS35 are widely used in research to study its expression, localization, and role in neurodegenerative diseases. Mutations in the *VPS35* gene (e.g., D620N) are linked to autosomal-dominant Parkinson’s disease, while disrupted retromer function is implicated in Alzheimer’s disease pathogenesis. These antibodies enable detection of VPS35 in techniques such as Western blotting, immunohistochemistry, and immunofluorescence, helping to explore its interaction partners and disease mechanisms.
Commercial VPS35 antibodies are typically raised against specific epitopes (human, mouse, or rat) and vary by clonality (monoclonal/polyclonal) and host species (rabbit, mouse). Validation includes testing in knockout models or siRNA-treated cells to confirm specificity, as cross-reactivity with unrelated proteins can occur. Researchers prioritize antibodies with proven performance in their experimental systems, as retromer dysregulation studies are pivotal for understanding intracellular trafficking defects and developing therapeutic strategies.
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