首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人1,4,5-三磷酸肌醇(IP3)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的IP3含量。IP3即三磷酸肌醇，是细胞内重要的第二信使。其背景在于它在细胞信号转导中扮演关键角色。研究机制上，当细胞外信号激活相应受体，通过G蛋白激活磷脂酶C，水解磷脂酰肌醇生成IP3，它能促使内质网释放Ca²⁺，引发一系列细胞反应。试剂盒检测范围为5 pg/mL-1000 pg/mL，尤其适用于体外研究细胞信号通路激活程度、药物靶点作用机制或疾病模型中IP3动态变化的分析；为探究G蛋白偶联受体介导的磷酸肌醇代谢、细胞应激反应调控等基础研究提供可靠工具，广泛适配于神经生物学、药理学及病理生理学等领域的科研需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：IP3
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：5 pg/mL-1000 pg/mL
灵敏度：2.5 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human IP3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 95 | ||||
| Range % | 90-105 | |||||
| 1:2 | Average % | 94 | ||||
| Range % | 89-100 | |||||
| 1:4 | Average % | 94 | ||||
| Range % | 87-102 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 90-102 | |||||
回收率：
| The recovery of human IP3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 91-100 | ||||
| EDTA plasma (n=4) | 92 | 87-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2Average 10000.2020.2110.207 2500.3500.3490.350 800.6000.5940.597 201.0291.0181.024 51.3261.3451.336 02.1022.1302.116 | ||||||
货期：3-5 working days
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