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产品描述：大鼠白介素12(IL-12/P70)酶联免疫试剂盒双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IL12/P70含量。IL12/P70是重要细胞因子，在免疫调节中发挥关键作用。它能激活T细胞和NK细胞来增强免疫应答。研究围绕其信号通路、与疾病关联机制展开，旨在通过调节它的活性开发治疗感染、肿瘤及自身免疫疾病的新疗法。试剂盒检测范围为3.12 pg/mL-200 pg/mL，该产品适用于科研场景中免疫机制研究，如炎症性疾病模型评估、疫苗免疫效果分析或药物干预后IL - 12/P70的动态变化观测，其中血清/血浆样本可用于系统性免疫状态分析，组织匀浆则适合局部炎症微环境研究。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：N/A
缩写：IL-12/P70
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：3.12 pg/mL-200 pg/mL
灵敏度：0.78 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-12/P70 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 96 | ||||
| Range % | 91-106 | |||||
| 1:2 | Average % | 94 | ||||
| Range % | 91-98 | |||||
| 1:4 | Average % | 93 | ||||
| Range % | 89-96 | |||||
| 1:8 | Average % | 87 | ||||
| Range % | 82-96 | |||||
回收率：
| The recovery of rat IL-12/P70 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 89-98 | ||||
| EDTA plasma (n=4) | 103 | 98-107 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected2002.5572.6562.6072.4251002.0232.1222.0731.891501.4481.4681.4581.276250.9280.9170.9230.74112.50.5780.5690.5740.3926.250.4150.4050.4100.2283.120.3030.3080.3060.12400.1810.1820.182 | ||||||
货期：3-5 working days
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